首页> 外国专利> IDENTIFICATION OF RNAI TARGETS AND USE OF RNAI FOR RATIONAL THERAPY OF CHEMOTHERAPY-RESISTANT LEUKEMIA AND OTHER CANCERS

IDENTIFICATION OF RNAI TARGETS AND USE OF RNAI FOR RATIONAL THERAPY OF CHEMOTHERAPY-RESISTANT LEUKEMIA AND OTHER CANCERS

机译:RNAi靶标的识别以及将RNAi用于抗化疗性白血病和其他癌症的合理治疗

摘要

Provided is a mosaic mouse model for use in determining the potency of an shRNA in vivo for reducing survival of cancer cells of chemotherapy-resistant leukemia. The syngeneic mouse recipient is transplanted with tet-on competent leukemia cells carrying a bicistronic nucleic acid construct comprising a promoter operably linked to a fusion gene associated with chemotherapy-resistant leukemia, and a sequence encoding a reverse tet-transactivator protein, such that both coding sequences are co-expressed from the promoter. Also provided are methods of treating soft tissue cancers.
机译:提供了一种镶嵌小鼠模型,用于确定shRNA在体内降低化疗耐药性白血病癌细胞的存活力的能力。将同基因小鼠受体移植到携带双顺反子核酸构建体的tet感受态白血病细胞中,该双顺反子核酸构建体包含可操作地连接至与化疗耐药性白血病相关的融合基因的启动子,以及编码反向tet-反式激活蛋白的序列,序列从启动子共表达。还提供了治疗软组织癌的方法。

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