首页> 外国专利> BARNACLE SPECIES DETERMINING PRIMER SET, AND METHOD FOR DETERMINING SPECIES OF BARNACLE LARVA AND METHOD FOR DETERMINING QUANTITY UTILIZING THE SAME

BARNACLE SPECIES DETERMINING PRIMER SET, AND METHOD FOR DETERMINING SPECIES OF BARNACLE LARVA AND METHOD FOR DETERMINING QUANTITY UTILIZING THE SAME

机译:细菌种类测定底漆集,测定细菌幼虫种类的方法和利用相同种类测定数量的方法

摘要

PROBLEM TO BE SOLVED: To easily and accurately determine species of barnacles in a nauplius larval stage and in a cypris larval stage and to quantitatively analyze the population of Northern barnacles such as Balanus rostratus, semibalanus cariosus and Balanus crenatus which can exist in a northern ocean area.SOLUTION: The species of a barnacle larva present in a sample is determined based on the presence of gene amplification of the sample by subjecting the sample to a PCR (polymerase chain reaction) process using a primer which specifically recognizes a specific area of 12S rRNA gene of each species of barnacle. A Ct value is obtained by the real-time PCR of the sample by using the primer which specifically recognizes 12S rRNA genes of each species of barnacle, and the relations between the population of barnacle larva and the Ct value are found by carrying out the real-time PCR process using the primer for each of a plurality of samples of species of barnacle larvae whose population is known. By utilizing a calibration curve prepared in advance, the populations of species of barnacle larvae present in the samples are quantitatively determined.
机译:要解决的问题:轻松,准确地确定无节幼体阶段和塞浦路斯幼体阶段的藤壶种类,并定量分析北部藤壶中的藤壶(如Balanus rostratus,semibalanus cariosus和Balanus crenatus)的种群解决方案:通过使用特异性识别12S特定区域的引物对样品进行PCR(聚合酶链反应)过程,根据样品中基因扩增的存在来确定样品中藤壶幼虫的种类。藤壶各物种的rRNA基因。通过使用特异性识别每种藤壶物种的12S rRNA基因的引物,通过实时PCR对样品进行Ct值测定,并通过实测来发现藤壶幼虫的种群与Ct值之间的关系。使用引物对已知种群的藤壶幼虫物种的多个样本中的每个样本进行实时PCR处理。通过利用预先准备的校准曲线,定量测定样品中存在的藤壶幼虫的种群。

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