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BARNACLE SPECIES DETERMINING PRIMER SET, AND METHOD FOR DETERMINING SPECIES OF BARNACLE LARVA AND METHOD FOR DETERMINING QUANTITY UTILIZING THE SAME
BARNACLE SPECIES DETERMINING PRIMER SET, AND METHOD FOR DETERMINING SPECIES OF BARNACLE LARVA AND METHOD FOR DETERMINING QUANTITY UTILIZING THE SAME
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机译:细菌种类测定底漆集,测定细菌幼虫种类的方法和利用相同种类测定数量的方法
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摘要
PROBLEM TO BE SOLVED: To easily and accurately determine species of barnacles in a nauplius larval stage and in a cypris larval stage and to quantitatively analyze the population of Northern barnacles such as Balanus rostratus, semibalanus cariosus and Balanus crenatus which can exist in a northern ocean area.SOLUTION: The species of a barnacle larva present in a sample is determined based on the presence of gene amplification of the sample by subjecting the sample to a PCR (polymerase chain reaction) process using a primer which specifically recognizes a specific area of 12S rRNA gene of each species of barnacle. A Ct value is obtained by the real-time PCR of the sample by using the primer which specifically recognizes 12S rRNA genes of each species of barnacle, and the relations between the population of barnacle larva and the Ct value are found by carrying out the real-time PCR process using the primer for each of a plurality of samples of species of barnacle larvae whose population is known. By utilizing a calibration curve prepared in advance, the populations of species of barnacle larvae present in the samples are quantitatively determined.
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