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PRIMER SET FOR IDENTIFYING BARNACLE SPECIES, METHOD FOR IDENTIFYING SPECIES OF BARNACLE LARVA, AND METHOD FOR QUANTIFYING THE BARNACLE LARVA
PRIMER SET FOR IDENTIFYING BARNACLE SPECIES, METHOD FOR IDENTIFYING SPECIES OF BARNACLE LARVA, AND METHOD FOR QUANTIFYING THE BARNACLE LARVA
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机译:用于鉴定细菌幼虫的底漆,用于鉴定细菌幼虫的方法以及用于定量细菌幼虫的方法
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摘要
PROBLEM TO BE SOLVED: To identify the species of barnacles in the nauplius larval stage and in the cypris larval stage, and to quantitatively analyze the number of individuals of the barnacles in the nauplius larval stage and in the cypris larval stage.;SOLUTION: A test sample is subjected to a PCR (polymerase chain reaction) using a primer pair specifically recognizing a specific region in 12S rRNA gene of the various barnacles to decide the species of barnacle larvae present in the test sample by the presence or absence of gene amplification of the test sample. The real-time PCR of the test sample is carried out by using the primer pair specifically recognizing the specific region in the 12S rRNA gene of the various barnacles to obtain a Ct (threshold cycle) value, and the real-time PCR is carried out for each of the plurality of samples in which the number of individuals of the barnacle larval species is known by using the primer pair to thereby investigate the relationship between the number of individuals of the barnacle larval species and the Ct value. As a result, the determination of the number of individuals of the barnacle larval species present in the test sample is made by utilizing a previously prepared calibration curve.;COPYRIGHT: (C)2010,JPO&INPIT
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