首页> 外国专利> PRIMER SET FOR IDENTIFYING BARNACLE SPECIES, METHOD FOR IDENTIFYING SPECIES OF BARNACLE LARVA, AND METHOD FOR QUANTIFYING THE BARNACLE LARVA

PRIMER SET FOR IDENTIFYING BARNACLE SPECIES, METHOD FOR IDENTIFYING SPECIES OF BARNACLE LARVA, AND METHOD FOR QUANTIFYING THE BARNACLE LARVA

机译:用于鉴定细菌幼虫的底漆,用于鉴定细菌幼虫的方法以及用于定量细菌幼虫的方法

摘要

PROBLEM TO BE SOLVED: To identify the species of barnacles in the nauplius larval stage and in the cypris larval stage, and to quantitatively analyze the number of individuals of the barnacles in the nauplius larval stage and in the cypris larval stage.;SOLUTION: A test sample is subjected to a PCR (polymerase chain reaction) using a primer pair specifically recognizing a specific region in 12S rRNA gene of the various barnacles to decide the species of barnacle larvae present in the test sample by the presence or absence of gene amplification of the test sample. The real-time PCR of the test sample is carried out by using the primer pair specifically recognizing the specific region in the 12S rRNA gene of the various barnacles to obtain a Ct (threshold cycle) value, and the real-time PCR is carried out for each of the plurality of samples in which the number of individuals of the barnacle larval species is known by using the primer pair to thereby investigate the relationship between the number of individuals of the barnacle larval species and the Ct value. As a result, the determination of the number of individuals of the barnacle larval species present in the test sample is made by utilizing a previously prepared calibration curve.;COPYRIGHT: (C)2010,JPO&INPIT
机译:要解决的问题:鉴定无节幼体阶段和幼体阶段的藤壶种类,并定量分析无节幼体阶段和lar幼体阶段的藤壶个体数量。使用特异性识别各种藤壶的12S rRNA基因中特定区域的引物对对样品进行PCR(聚合酶链反应),以通过存在或不存在以下基因的基因扩增来确定存在于样品中的藤壶幼虫的种类。测试样本。通过使用特异性识别各种藤壶的12S rRNA基因中特定区域的引物对进行测试样品的实时PCR,以获得Ct(阈值循环)值,并进行实时PCR对于其中通过使用引物对已知藤壶幼虫物种的个体数目的多个样品中的每一个,从而研究藤壶幼虫物种的个体数目与Ct值之间的关系。结果,通过使用预先准备的校准曲线确定了存在于测试样品中的藤壶幼虫物种的个体数。;版权所有:(C)2010,JPO&INPIT

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