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METHOD FOR IMMUNOENZYMOMETRIC DETECTION OF T-2 MYCOTOXIN IN LIQUID SAMPLES

机译：液体样品中T-2霉菌毒素的免疫比色法检测

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FIELD: chemistry.;SUBSTANCE: during immunoenzymometric analysis, a solid-phase antigen is immobilised on the surface of a solid phase. Further, a sample and a preparation which contains specific antigens with which free mycotoxin and the solid phase compete to bind are added. An anti-specific immunoenzymometric conjugate is then added and finally results are evaluated using a substrate-indicator mixture. The solid-phase antigen used is a conjugate of T-2 mycotoxin with an antigen of fraction 1 of a pestilential microbe. Rabbit hyperimmune serum is used at the competitive binding step.;EFFECT: invention increases sensitivity and specificity during detection of T-2 mycotoxin in liquid samples.;2 ex

机译：领域：化学。物质：在免疫酶分析中，固相抗原被固定在固相表面上。此外，加入含有特异性抗原的样品和制剂，游离的真菌毒素和固相竞争与之结合。然后加入抗特异性免疫酶联缀物，最后使用底物-指示剂混合物评估结果。所用的固相抗原是T-2霉菌毒素与瘟疫微生物级分1的抗原的结合物。兔超免疫血清用于竞争性结合步骤。效果：本发明提高了检测液体样品中T-2霉菌毒素的敏感性和特异性。

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公开/公告号RU2412443C1

专利类型
公开/公告日2011-02-20

原文格式PDF
申请/专利权人 FEDERALNOE GOSUDARSTVENNOE UCHREZHDENIE 48 TSENTRALNYJ NAUCHNO-ISSLEDOVATELSKIJ INSTITUT MINISTERSTVA OBORONY ROSSIJSKOJ FEDERATSII;
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申请/专利号RU20090133506
发明设计人 DUBROVIN MIKHAIL JUREVICH;KYTMANOV ALEKSEJ ALEKSANDROVICH;DARMOV ILJA VLADIMIROVICH;BAKULINA LJUDMILA VLADIMIROVNA;VOROBEV KONSTANTIN ANATOLEVICH;
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申请日2009-09-07
分类号G01N33/53;C12Q1/28;
国家 RU
入库时间 2022-08-21 17:48:31

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