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Haplotype Marker Single Base Variation and Uses thereof for the Functional Variation Analysis of Pregnan X Receptor Genes
Haplotype Marker Single Base Variation and Uses thereof for the Functional Variation Analysis of Pregnan X Receptor Genes
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机译:单倍型标记单碱基变异及其在Pregnan X受体基因功能变异分析中的应用
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摘要
A method for selecting htSNP of a genotype of PXR gene is provided to confirm the functional genotype of PXR gene discovered in the Asian which is similar to Korean genetic character easily by using a htSNP obtained with the SNP of the PXR gene of Korean. A method for selecting htSNP of a genotype of PXR gene comprises (1) a step for collecting the biological material from the human; (2) a step for extracting a nucleic acid from the collected sample of the step (1); (3) a step for performing PCR with a primer which amplifies the human PXR gene or its fraction by using a nucleic acid of the step (2) as a template; (4) a step for confirming the variation in the sequence of the PCR product obtained from the step (3); (5) a step for analyzing the haplotype in the sequence of the PCR product which exists variation in the step (4); and (6) a step for selecting htSNP by analyzing the sequence of the haplotype analyzed in the step (5) by using the SNP tagger software.
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