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METHOD FOR PRODUCING HUMAN EMBRYONIC STEM CELL IN CULTURE SYSTEM WITHOUT COMPONENTS DERIVED FROM ANIMAL AND HUMAN EMBRYONIC STEM CELL BY PRODUCED THEREOF
METHOD FOR PRODUCING HUMAN EMBRYONIC STEM CELL IN CULTURE SYSTEM WITHOUT COMPONENTS DERIVED FROM ANIMAL AND HUMAN EMBRYONIC STEM CELL BY PRODUCED THEREOF
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机译：不含动物和人胚胎干细胞成分的培养系统中人胚胎干细胞的生产方法
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摘要
PURPOSE: A method for preparing human embryonic stem cells without components derived from animal is provided to differentiate the stem cells to various organs. CONSTITUTION: A method for preparing human embryonic stem cells in a culture system without components derived from animal comprises: a step of culturing human foreskin cells(ATCC, CRL-2429, USA) into a feeder layer using humanized CELLstart; a step of performing co-culturing an embryo of human 4-cell stage with the feeder layer; a step of removing zona pellucida using Tyrode's solution; a step of removing trophoblase by mechanical dissection to isolate an inner cell mass(ICM); a step of performing co-culture of the ICM on the feeder layer in a medium containing 15% knockout SR xeno-feee, 8ng/ml of bFGF; and a step of isolating ICM and performing co-culture of the ICM dome with the feeder layer.
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机译：目的：提供一种无人源成分的人胚胎干细胞的制备方法，以将干细胞分化为各种器官。组成：一种在没有来自动物的成分的培养系统中制备人胚胎干细胞的方法，包括：使用人源化的CELLstart将人包皮细胞（ATCC，CRL-2429，USA）培养到饲养层中的步骤;将人4细胞期的胚胎与饲养层共同培养的步骤;用泰罗德溶液去除透明带的步骤;通过机械解剖去除滋养酶的步骤以分离内部细胞团（ICM）;在含有15％敲除SR异种费，8ng / ml bFGF的培养基中在饲养层上进行ICM共培养的步骤;隔离ICM并与饲养层进行ICM圆顶共培养的步骤。
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