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Hbridación mark and method for rapid detection and sequence discrimination.
Hbridación mark and method for rapid detection and sequence discrimination.
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机译：Hbridación标记以及用于快速检测和序列识别的方法。
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摘要
A hybridisation beacon which is an oligonucleotide where (a) the oligonucleotide has no secondary structure arising from one region of the oligonucleotide which hybridizes with another, (b) the oligonucleotide is formed of nucleotide residues of which more than one is labeled with a fluorescent reporter wherein the beacon does not include a fire associated and the indicator is selected from the group of 6-carboxyfluorescein, tetrachlorofluorescein and hexachlorofluorescein, (c) the nucleotide residues labeled indicator are positioned internally within the oligonucleotide sequence, (d ) the oligonucleotide is fully complementary to one allele of human genomic DNA having a polymorphism known in the binding site of the oligonucleotide, and (e) the oligonucleotide probe is modified at its 3 'end to prevent chain extension during PCR amplification.
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