首页>
外国专利>
vector, host cell, kit, composition of cell culture supplements, and methods for selecting cells that can survive in a cholesterol free medium to obtain cells that have the ability to survive in a cholesterol free medium and produce a heterologous protein, and to express a heterologous protein
vector, host cell, kit, composition of cell culture supplements, and methods for selecting cells that can survive in a cholesterol free medium to obtain cells that have the ability to survive in a cholesterol free medium and produce a heterologous protein, and to express a heterologous protein
VECTOR, HOST CELL, KIT, COMPOSITION OF CELL CULTURE SUPPLEMENTS, AND, METHODS FOR SELECTING CELLS WHICH CAN SURVIVE UNDER COLESTEROL, TO OBTAIN CELLS HAVING THE SURVIVAL HANDLING AND TO EXPRESS A Heterologous Protein. The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells. More specifically, the present invention provides compositions and methods that utilize any enzyme useful in the eukaryotic sterol / cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker for selecting transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in cholesterol-deficient medium and / or of producing the heterologous protein in these cells in chemically defined and / or serum free media.
展开▼
