首页> 外国专利> Genome-Wide Construction of Schizosaccharomyces Pombe Heterozygous Deletion Mutants Containing Gene-Specific Barcodes by the Methods of 4-Round Serial or Block PCR, or Total Gene Synthesis Thereof

Genome-Wide Construction of Schizosaccharomyces Pombe Heterozygous Deletion Mutants Containing Gene-Specific Barcodes by the Methods of 4-Round Serial or Block PCR, or Total Gene Synthesis Thereof

机译：通过四轮串联或嵌段PCR或全基因合成方法构建含基因特异性条形码的粟酒裂殖酵母杂合子缺失突变体的全基因组构建

页面导航

摘要
著录项
相似文献

摘要

A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.

机译：提供了一种方法，该方法包括通过缺失盒转化粟酒裂殖酵母的方法，所述缺失盒通过四轮连续PCR，嵌段PCR或总基因合成而构建，其包含同源重组位点，用于制备基因靶向的杂合缺失。粟酒裂殖酵母。还提供了通过该方法制备的针对基因的杂合缺失粟酒裂殖酵母突变体，以及提供了针对基因的杂合缺失粟酒裂殖酵母突变体的文库。此外，该文库可用于构建筛选药物作用方式的方法和试剂盒。 展开▼

著录项

公开/公告号US2011190163A1

专利类型

公开/公告日2011-08-04

原文格式PDF

申请/专利权人 KWANG LAE HOE;DONG UK KIM;MI SUN WON;HYANG SOOK YOO;DONG SUP KIM;HAN OH PARK;KYUNG SOOK CHUNG;YOUNG JOO JANG;MI YOUNG NAM;SANG JO HAN;HIN JUNG CHOI;SEUNG TAE BAEK;HYONG BAI KIM;KYUNG SUN HEO;HYE MI LEE;MIN HO LEE;JO YOUNG PARK;
展开▼

申请/专利号US20080989192

发明设计人 MIN HO LEE;JO YOUNG PARK;KWANG LAE HOE;DONG SUP KIM;HAN OH PARK;HYE MI LEE;MI YOUNG NAM;MI SUN WON;HIN JUNG CHOI;YOUNG JOO JANG;SEUNG TAE BAEK;HYANG SOOK YOO;DONG UK KIM;KYUNG SOOK CHUNG;HYONG BAI KIM;SANG JO HAN;KYUNG SUN HEO;
展开▼

申请日2008-08-27

分类号C40B30/06;C12N1/19;

国家 US

入库时间 2022-08-21 18:12:32

相似文献

专利

外文文献

中文文献

1. 通过4轮连续PCR或阻断PCR或其全基因合成的方法进行的含有基因特异的条形码的粟酒裂殖酵母杂合缺失突变体的全基因组构建 [P] . 中国专利： CN102016022B . 2014.05.14

2. 通过4轮连续PCR或阻断PCR或其全基因合成的方法进行的含有基因特异的条形码的粟酒裂殖酵母杂合缺失突变体的全基因组构建 [P] . 中国专利： CN102016022A . 2011-04-13

3. Genome-wide construction of Schizosaccharomyces pombe heterozygous deletion mutants containing gene-specific barcodes by the methods of 4-round serial or block PCR, or total gene synthesis thereof [P] . 美国专利： US9670480B2 . 2017-06-06

机译：通过四轮连续或嵌段PCR或全基因合成的方法构建全基因的粟酒裂殖酵母杂合子缺失突变体全基因组构建

4. Genome-wide construction of Schizosaccharomyces pombe heterozygous deletion mutants containing gene-specific barcodes by the methods of 4-round serial or block PCR, or total gene synthesis thereof [P] . 韩国专利： KR101098032B1 . 2011-12-23

机译：通过四轮串联或嵌段PCR方法或其全基因合成方法构建全基因组含基因特异性条形码的粟酒裂殖酵母杂合缺失突变体

5. GENOME-WIDE CONSTRUCTION OF SCHIZOSACCHAROMYCES POMBE HETEROZYGOUS DELETION MUTANTS CONTAINING GENE-SPECIFIC BARCODES BY THE METHODS OF 4-ROUND SERIAL OR BLOCK PCR, OR TOTAL GENE SYNTHESIS THEREOF [P] . 欧洲知识产权局专利： EP2268808A4 . 2011-09-14

机译：通过四轮串联或嵌段PCR或全基因合成的方法构建包含基因特异性条形码的裂殖酵母球菌多态性缺失突变体

1. A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length [J] . Ning-Ning Liu, Tian Xu Han, Li-Lin Du, 细胞研究（英文版） . 2010,第008期

2. Generation of gua1 deletion using polymerase chain reaction (PCR)-mediated gene disruption method in fission yeast, Schizosaccharomyces pombe [J] . Merve YILMAZER, Bedia PALABIYIK, Aysegul TOPAL SARIKAYA, African Journal of Biotechnology . 2017,第27期

3. A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length [J] . 无细胞研究：英文版 . 2010,第008期

4. Discovery of genes involved in mitosis cell division cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains [O] . Jun-Song Chen, Janel R. Beckley, Liping Ren, -1

5. Generation of gua1 deletion using polymerase chain reaction (PCR)-mediated gene disruption method in fission yeast, Schizosaccharomyces pombe [O] . YILMAZER Merve, PALABIYIK Bedia, TOPAL SARIKAYA Aysegul, 2017

1. 长叶车前花叶病毒上海分离物(RMV sh)基因组全序列的测定,侵染性克隆的构建及外源基因的表达 [C] . 朱洪庆 ,于善谦 ,陈剑平 . 中国植物病理学会2004年学术年会 . 2004

2. 鹅源新城疫病毒ZJ1株全基因组序列的测定及含该毒株全基因组cDNA的转录载体的构建 [A] . 黄勇 . 2003