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PROCESS FOR PRODUCING ALPHA-GLYCOSYLATED DIPEPTIDE AND METHOD OF ASSAYING ALPHA-GLYCOSYLATED DIPEPTIDE

机译:制备α-糖基化的二肽的方法和测定α-糖基化的二肽的方法

摘要

The present invention relates to a method for producing α-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of α-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the α-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing α-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of α-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of α-glycated dipeptide is provided, which enables to determine the amount of α-glycated dipeptide in a highly precise manner within a short time period.
机译:本发明涉及生产α-糖基化二肽的方法,该方法包括使蛋白酶作用于N-末端糖基化的肽或N-末端糖基化的蛋白质。本发明还涉及确定α-糖基化二肽的量的方法,该方法包括使果糖基肽氧化酶作用于通过上述方法获得的α-糖基化二肽,然后确定由此产生的过氧化氢的量。根据本发明,提供了一种生产α-糖基化二肽的方法,该方法能够从糖化蛋白或糖基化肽简单,快速和有效地生产α-糖化二肽。此外,根据本发明,提供了一种确定α-糖基化二肽的量的方法,该方法使得能够在短时间内高精度地确定α-糖基化二肽的量。

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