首页> 外国专利> PROCESS FOR PRODUCING a-GLYCOSYLATED DIPEPTIDE AND METHOD OF ASSAYING a-GLYCOSYLATED DIPEPTIDE

PROCESS FOR PRODUCING a-GLYCOSYLATED DIPEPTIDE AND METHOD OF ASSAYING a-GLYCOSYLATED DIPEPTIDE

机译:α-糖基化二肽的生产方法和评估α-糖基化二肽的方法

摘要

The present invention relates to a method for producing ±-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of ±-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the ±-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing ±-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of ±-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of ±-glycated dipeptide is provided, which enables to determine the amount of ±-glycated dipeptide in a highly precise manner within a short time period.
机译:本发明涉及生产β-糖基化二肽的方法,该方法包括使蛋白酶作用于N-末端糖基化的肽或N-末端糖基化的蛋白质。本发明还涉及确定±糖基化二肽的量的方法,该方法包括使果糖基肽氧化酶作用于通过上述方法获得的±糖基化二肽,然后确定由此产生的过氧化氢的量。根据本发明,提供了一种生产±糖化二肽的方法,该方法使得能够从糖化蛋白或糖化肽简单,快速和有效地生产±糖化二肽。此外,根据本发明,提供了一种确定±糖化二肽的量的方法,该方法使得能够在短时间内高精度地确定±糖化二肽的量。

著录项

  • 公开/公告号EP1679378A4

    专利类型

  • 公开/公告日2010-05-19

    原文格式PDF

  • 申请/专利权人 KIKKOMAN CORPORATION;

    申请/专利号EP20040772984

  • 申请日2004-09-13

  • 分类号C12P21/06;C12Q1/26;C12P21/02;C12Q1/37;

  • 国家 EP

  • 入库时间 2022-08-21 18:39:28

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