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Being the manner which decides the transplantation aptitude decision manner, and quality control manner of culture cartilage system and the transplantation aptitude of production mannered null

机译:作为决定移植能力决定方式的方式,培养软骨体系的质量控制方式和生产移植能力的决定方式无效。

摘要

PROBLEM TO BE SOLVED: To objectively and efficiently decide suitability for transplantation of a cultured tissue and to efficiently supply the cultured tissue having a stable quality by an objective criterion. SOLUTION: A pH value of 6.9 corresponding to 1×106 cultured cartilage cells/ml is set to a criterial pH value in a cultured cartilaginous tissue. The decision that the cultured cartilaginous tissue has suitability for transplantation is drawn when the pH value measured after starting culturing is less than 6.9 because the number of the cells and the production amount of a substrate increase and the pH value decreases accompanied with a lapse of a culture period in the cultured cartilaginous tissue. The quality is controlled by regulating the culture period, or the like, based on the comparison with the criterial pH value.
机译:要解决的问题:客观有效地确定培养组织移植的适合性,并通过客观标准有效地提供具有稳定质量的培养组织。溶液:将对应于1×10 6培养的软骨细胞/ ml的6.9的pH值设置为培养的软骨组织中的标准pH值。当开始培养后测得的pH值小于6.9时,由于细胞数和底物的产生量增加,且pH值降低,伴随着时间的流逝,得出培养的软骨组织适合移植的判断。培养的软骨组织中的培养期。基于与标准pH值的比较,通过调节培养时间等来控制质量。

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