首页> 外国专利> MICROFLUIDIC DEVICES AND METHODS FOR PROTEINS CRYSTALLIZATION AND IN SITU X-RAY DIFFRACTION

MICROFLUIDIC DEVICES AND METHODS FOR PROTEINS CRYSTALLIZATION AND IN SITU X-RAY DIFFRACTION

机译:蛋白质结晶和原位X射线衍射的微流体装置和方法

摘要

The present disclosure is directed generally to devices and methods with the purpose of interfacing micro fluidic devices with dispensing and fluid handling systems to achieve the rapid identification of protein crystallization conditions. The device described herein is fabricated with the use of a cyclic olefin homopolymer-based creating microfluidics system adaptable for protein crystallization and in situ X-ray diffraction. Connectivity between chambers is controlled by valves that allow specified volumes of liquid to be transferred from one chamber to another. The microfluidic system is useful to established microbatch, vapor diffusion and free interface diffusion protocols for protein crystallization and to obtain crystals for a number of proteins, including chicken lysozyme, bovine trypsin, a human p53 protein containing both the DNA binding and oligomerization domains bound to DNA and a functionally important domain of Arabidopsis Morpheus' Molecule 1 (MOMl). For X-ray diffraction analysis, either the microfluidic devices were opened to allow mounting of the crystals on loops or the crystals were exposed to X-rays in situ. Thus, cyclic olefin homopolymer-based microfluidics systems are useful to further automate protein crystallization and structural genomics efforts.
机译:本公开总体上针对旨在使微流体装置与分配和流体处理系统对接以实现蛋白质结晶条件的快速鉴定的装置和方法。本文所述的装置是使用基于环烯烃均聚物的产生微流体系统制造的,该系统适用于蛋白质结晶和原位X射线衍射。腔室之间的连通性由阀门控制,这些阀门允许将指定体积的液体从一个腔室转移到另一个腔室。微流体系统可用于建立蛋白质结晶的微批处理,蒸气扩散和自由界面扩散方案,以及获得多种蛋白质的晶体,包括鸡溶菌酶,牛胰蛋白酶,人p53蛋白质,该蛋白质同时包含与DNA结合和寡聚化域DNA和拟南芥Morpheus分子1(MOM1)的功能重要结构域。对于X射线衍射分析,要么打开微流控设备以允许将晶体安装在环上,要么将晶体原位暴露于X射线。因此,基于环状烯烃均聚物的微流体系统可用于进一步自动化蛋白质结晶和结构基因组学研究。

著录项

  • 公开/公告号WO2009150549A2

    专利类型

  • 公开/公告日2009-12-17

    原文格式PDF

  • 申请/专利权人 SPINX INC.;ZUCCHELLI PIERO;

    申请/专利号WO2009IB06586

  • 发明设计人 ZUCCHELLI PIERO;

    申请日2009-06-15

  • 分类号B01L3/06;B01L3/00;B01D9/00;C30B7/00;C30B29/58;

  • 国家 WO

  • 入库时间 2022-08-21 18:40:43

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