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system for cell and developmental selection differentiated embryonic stem cells, adult stem cells and embryonic keinbahnzellen

机译:细胞和发育选择系统分化的胚胎干细胞,成年干细胞和胚胎keinbahnzellen

摘要

Embryonic stem cells, embryonic germ-line cells and/or adult stem cells (A) that contain at least one each of reporter gene (RG1) and resistance gene (RG2) under control of the same promoter and present in at least one or two vector constructs. The promoter is cell specific and/or development specific. Independent claims are also included for the following: (a) differentiating or differentiated cells (B) produced from (A); (b) method for producing differentiating (A); (c) cultures (C) of (A) or (B); (d) method (D) for toxicological testing using the cultures (C); (e) method (E) for generating transgenic non-human animals using (A) or (B); (f) transgenic animals produced by method (E); (g) method for testing the developmental stage of mammalian cells; and (h) differentiated cells, especially of the heart, prepared from (A) or (B).
机译:胚胎干细胞,胚胎生殖细胞和/或成体干细胞(A),它们在同一启动子的控制下分别含有报告基因(RG1)和抗性基因(RG2)中的至少一个,并且存在至少一个或两个向量构建体。启动子是细胞特异性和/或发育特异性的。还包括以下方面的独立权利要求:(a)从(A)产生的分化或分化的细胞(B); (b)产生差异的方法(A); (c)(A)或(B)的文化(C); (d)使用培养物(C)进行毒理学测试的方法(D); (e)使用(A)或(B)产生转基因非人类动物的方法(E); (f)通过方法(E)产生的转基因动物; (g)测试哺乳动物细胞发育阶段的方法; (h)由(A)或(B)制备的分化细胞,特别是心脏细胞。

著录项

  • 公开/公告号AT475705T

    专利类型

  • 公开/公告日2010-08-15

    原文格式PDF

  • 申请/专利权人 AXIOGENESIS AG;

    申请/专利号AT20010272041T

  • 申请日2001-12-27

  • 分类号C12N5/0735;A01K67/027;A61K35/545;A61L27/00;C12N5/077;C12N5/10;C12N15/09;C12N15/85;C12Q1/02;G01N33/15;G01N33/50;

  • 国家 AT

  • 入库时间 2022-08-21 18:43:37

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