首页> 外国专利> Recombined glycoproteins, DNA molecules, biologically active vector, method for cDNA obtaining, method for GlcNAc 1, 3-fucosyltransferase cloning, method for obtaining recombined host cells, method for obtaining recombined plants, recombined plants and plant cells, recombined insects and insect cells, peptide nucleid acid molecules, method for obtaining plants or insects of cells thereof with blocked expression of GlcNAc 1, 3-fucosyltransferase, method for obtaining recombined glycoproteins and human glycoproteins, reco

Recombined glycoproteins, DNA molecules, biologically active vector, method for cDNA obtaining, method for GlcNAc 1, 3-fucosyltransferase cloning, method for obtaining recombined host cells, method for obtaining recombined plants, recombined plants and plant cells, recombined insects and insect cells, peptide nucleid acid molecules, method for obtaining plants or insects of cells thereof with blocked expression of GlcNAc 1, 3-fucosyltransferase, method for obtaining recombined glycoproteins and human glycoproteins, reco

机译：重组糖蛋白，DNA分子，生物活性载体，cDNA的获得方法，GlcNAc 1、3-岩藻糖基转移酶的克隆方法，重组宿主细胞的获得方法，重组植物，重组植物和植物细胞的获得方法，昆虫和昆虫细胞的重组，肽核酸分子，获得GlcNAc 1，3-岩藻糖基转移酶表达受阻的植物或昆虫的方法，重组糖蛋白和人糖蛋白的获得方法，reco

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摘要

A DNA molecule (I), encoding a plant protein with fucosyl transferase activity, comprising a 2198 base pair sequence, fully defined in the specification, with an open reading frame of nucleotides 211-1740, a sequence at least 50 % homologous to it, a sequence hybridizing to it under stringent conditions, a sequence which differs due to genetic code degeneracy, or their complement, is new. Independent claims are also included for the following: (1) a DNA molecule (II), comprising a 105 base pair sequence, fully defined in the specification, a sequence at least 95 % homologous to it, a sequence that hybridizes to it under stringent conditions, or a sequence which differs due to genetic code degeneracy; (2) a DNA molecule which is a partial sequence of (I) or (II), which comprises 30-50 base pairs; (3) (I) or (II) covalently associated with a detectable marker substance; (4) a biologically functional vector comprising (I) or (II) or a partial sequence of at least 20 base pairs; (5) a biologically functional vector comprising (I), (II) or a partial sequence in inverse orientation to the promoter; (6) (I), (II) or the DNA molecule of (2) or (3) comprising a deletion, insertion and/or substitution mutation; (7) a DNA encoding a ribozyme, which comprises two sequence sections of at least 10-15 base pairs, which are complementary to (I) or (II), so that the ribozyme complexes with and cuts the mRNA, which is transcribed from a natural GlcNAc-alpha 1,3-fucosyl transferase DNA molecule; (8) a biologically functional vector comprising DNA of (6) or (7); (9) producing a cDNA of (I) or (II) comprising isolating RNA of insects or plants, and carrying out reverse transcription with a reverse transcriptase and primers; (10) cloning a GlcNAc-alpha 1,3-fucosyl transferase; (11) producing recombinant host cells which over-express GlcNAc-alpha 1,3-fucosyltransferase; (12) producing recombinant host cells, preferably plant or insect cells, which have a DNA of (6) in place of the non-mutated homologous sequence; (13) a recombinant plant or insect cell produced by the method of (12) and which over-expresses the GlcNAc-alpha 1,3-fucosyltransferase; (14) a PNA (peptide-nucleic acid) molecule which is complementary to or the same as (I); (15) producing a plant or insect cell with blocked GlcNAc-alpha 1,3-fucosyl transferase expression at the level of transcription or translation by the PNA of (14); (16) a method of recombinant glycoprotein production; (17) a recombinant glycoprotein produced in a plant or insect cell system as above; a GlcNAc-alpha 1,3-fucosyltransferase preparation, cloned by a method of (16), where the isoforms have a pI of between 6 and 9, preferably 6.8 and 8.2; and (18) producing human and other vertebrate carbohydrates by transplantation.

机译：编码具有岩藻糖基转移酶活性的植物蛋白的DNA分子（I），包含规范中完全定义的2198个碱基对序列，并具有核苷酸211-1740的开放阅读框，与该序列至少50％同源，在严格条件下与其杂交的序列，由于遗传密码简并性或其互补而不同的序列是新的。还包括以下方面的独立权利要求：（1）DNA分子（II），包括在说明书中完整定义的105个碱基对的序列，与之至少95％同源的序列，在严格条件下与其杂交的序列条件或由于遗传密码简并性而不同的序列; （2）是（I）或（II）的部分序列的DNA分子，其包含30-50个碱基对; （3）（I）或（II）与可检测的标记物共价结合; （4）包含（I）或（II）或至少20个碱基对的部分序列的生物学功能载体; （5）生物功能载体，其包含与启动子相反方向的（I），（II）或部分序列。（6）（I），（II）或（2）或（3）的DNA分子，其包含缺失，插入和/或取代突变; （7）编码核酶的DNA，其包含与（I）或（II）互补的至少10-15个碱基对的两个序列部分，从而核酶与mRNA复合并切割mRNA，该mRNA从天然的GlcNAc-α1,3-岩藻糖基转移酶DNA分子; （8）一种生物功能载体，其包含（6）或（7）的DNA。（9）产生（I）或（II）的cDNA，其包括分离昆虫或植物的RNA，并用逆转录酶和引物进行逆转录; （10）克隆GlcNAc-α1,3-岩藻糖基转移酶; （11）产生过表达GlcNAc-α1，3-岩藻糖基转移酶的重组宿主细胞; （12）产生重组宿主细胞，优选植物或昆虫细胞，其具有（6）的DNA代替非突变的同源序列; （13）通过（12）的方法产生的重组植物或昆虫细胞，其过表达GlcNAc-α1,3-岩藻糖基转移酶。（14）与（I）互补或相同的PNA（肽-核酸）分子; （15）产生一种植物或昆虫细胞，其在（14）的PNA的转录或翻译水平上具有被阻断的GlcNAc-α1,3-岩藻糖基转移酶表达; （16）重组糖蛋白生产的方法; （17）在上述植物或昆虫细胞系统中产生的重组糖蛋白;通过（16）的方法克隆的GlcNAc-α1,3-岩藻糖基转移酶制剂，其中同工型的pI在6至9之间，优选在6.8至8.2之间; （18）通过移植产生人和其他脊椎动物的碳水化合物。

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公开/公告号PL205710B1

专利类型
公开/公告日2010-05-31

原文格式PDF
申请/专利权人 ALTMANN FRIEDRICH;
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申请/专利号PL20000385768
发明设计人 ALTMANN FRIDRICH;
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申请日2000-02-17
分类号A01H5;C12N15/54;A01K67/033;A61K38/45;A61P37/08;A61P43;C12N;C12N5/10;C12N9;C12N9/10;C12N15/09;C12N15/11;C12P;C12P19/18;C12P19/44;C12P21;C12Q1/68;
国家 PL
入库时间 2022-08-21 18:45:15

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