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SUPPRESSION OF RESISTANCE IN INSECTS TO BACILLUS THURINGIENSIS CRY TOXINS, USING TOXINS THAT DO NOT REQUIRE THE CADHERIN RECEPTOR.
SUPPRESSION OF RESISTANCE IN INSECTS TO BACILLUS THURINGIENSIS CRY TOXINS, USING TOXINS THAT DO NOT REQUIRE THE CADHERIN RECEPTOR.
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机译：使用不需要钙黏着蛋白受体的毒素来抑制对苏云金芽孢杆菌Cry毒素的抗性。
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摘要
The invention relates to a method for obtaining DNA constructions coding for Cry toxins with 3 domains (also known as Cry toxins, Bt toxins or δ-endotoxins), which lack helix α-1. Said DNA constructions were modified in order to encode proteins that can kill insects that are resistant to the corresponding non-modified Cry toxins. The invention relates to the DNA constructions of the genes of modified 3-domain Cry toxins and the modified 3-domain Cry proteins, together with methods, molecular vectors and host cells containing said constructions, as well as recombinant methods for producing the modified 3-domain Cry toxins. In addition, the invention relates to formulations containing the modified 3-domain Cry toxins. Resistance of insects to non-modified Cry toxins is due to a reduced binding of the toxin to the receptors in the insect's intestine. More specifically, the invention relates to the expression of the modified 3-domain Cry toxins, the methods for expressing said modified 3-domain Cry toxins, transformed microorganisms and transgenic plants with constructions that express the modified 3-domain Cry toxins and methods for suppressing resistance of pests to non-modified Cry toxins.
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