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CONFIGURATION OF A LASER SCANNING MICROSCOPE FOR RASTER IMAGE CORRELATION SPECTROSCOPY MEASUREMENT AND METHOD FOR CONDUCTING AND EVALUATING SUCH A MEASUREMENT
CONFIGURATION OF A LASER SCANNING MICROSCOPE FOR RASTER IMAGE CORRELATION SPECTROSCOPY MEASUREMENT AND METHOD FOR CONDUCTING AND EVALUATING SUCH A MEASUREMENT
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机译：用于光栅图像相关光谱测量的激光扫描显微镜的配置以及进行和评估这种测量的方法
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摘要
1.1. Method for the configuration a laser scanning microscope for a raster image correlation spectroscopy measurement and method for carrying out and evaluating a measurement of this kind. 2.1. Manual setting of the scan parameters for a raster image correlation spectroscopy measurement (RICS) is complicated because the effects of setting a certain parameter are not apparent due to the complex interaction between the various parameters and also depend on the physical-technical properties of the microscope. By means of an improved configuration method, mathematical transport models can be fitted to correlations determined by means of scanning fluorescence spectroscopy with few errors. With improved methods for carrying out or evaluating a RICS measurement, the amount of data to be stored can be reduced and RICS correlations of high statistical quality can be determined within a short period of time. 2.2. According to the invention, for a raster image correlation spectroscopy measurement, a best value for a sampling value is determined and is specified for a subsequent scanning process on a sample. In order to carry out or evaluate a RICS measurement, sampling values are acquired or a correlation is determined exclusively in a sample region within which a pixel time (ΔP) changes along a harmonically controlled scan axis (X) by less than, or at most by, a predetermined or predeterminable value. 2.3. The invention is preferably used in laser scanning microscopes.
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