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Atopic dermatitis mouse and the screening method for protective agent, treatment agnent or cosmetic composition of atopic dermatitis using thereof
Atopic dermatitis mouse and the screening method for protective agent, treatment agnent or cosmetic composition of atopic dermatitis using thereof
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机译:特应性皮炎小鼠及其使用的特应性皮炎的保护剂,治疗表皮或化妆品组合物的筛选方法
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摘要
A method of screening a cosmetic composition is provided to prevent or cure the atopic dermatitis using an atopic dermatitis mouse. A method of manufacturing an atopic dermatitis mouse comprises steps of: 1) dissolving 50 ~ 100 mul of ovalbumin in a 50 ~ 100 mul of alum, intraperitoneal-injecting it in a mouse, dissolving the 50 ~ 100 mul of ovalbumin in a 50 ~ 100 mul of PBS and epicutaneous-sensitizing it for 6 ~ 8 days; 2) finishing the epicutaneous sensitization of the step 1), dissolving the 50 ~ 100 mul of ovalbumin in the 50 ~ 100 mul of PBS after 13 ~ 15 days, intraperitoneal-injecting it in the mouse, dissolving the 50 ~ 100 mul of ovalbumin in the 50 ~ 100 mul of PBS and epicutaneous-sensitizing it for 6 ~ 8 days.
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机译:提供了使用特应性皮炎小鼠筛选化妆品组合物以预防或治愈特应性皮炎的方法。制造特应性皮炎小鼠的方法包括以下步骤:1)将50〜100 mul卵清蛋白溶解在50〜100 mul矾中,将其腹膜内注射到小鼠中,将50〜100 mul卵清蛋白溶解在50〜100 mul中。 100 ul PBS,并对其进行表皮敏化6〜8天; 2)完成步骤1)的表皮敏化,在13〜15天后将50〜100 mul卵清蛋白溶解在50〜100 mul PBS中,将其腹膜内注射到小鼠体内,溶解50〜100 mul卵清蛋白在50〜100 mul的PBS中进行表皮敏化6〜8天。
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