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METHOD FOR PREPARATION OF HEPATOCYTES WITH LIVER-SPECIFIC FUNCTIONS USING MESENCHYMAL STEM CELLS AND UMBILICAL CORD-DERIVED HEPATOCYTES PREPARED THEREBY
METHOD FOR PREPARATION OF HEPATOCYTES WITH LIVER-SPECIFIC FUNCTIONS USING MESENCHYMAL STEM CELLS AND UMBILICAL CORD-DERIVED HEPATOCYTES PREPARED THEREBY
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机译:用间充质干细胞和脐带来源的肝细胞制备具有肝功能的肝细胞的方法
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摘要
A method for manufacturing mesenchymal stem cell derived hepatocyte is provided to differentiate a mesenchymal stem cell which is derived from a wharton's jelly. A method for manufacturing mesenchymal stem cell derived hepatocyte comprises: a step of culturing the mesenchymal stem cell in cell proliferation medium; a step of inducing cells to hepatocyte in serum medium in which cell growth factor is added; and a step of maturing hepatocyte in serum medium in which a hepatocyte maturing induction material is added. The hepatocyte maturing induction material is selected from an oncostatin M, glucagon, nicotin amide and dimethyl sulfoxid(DMSO).
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