首页> 外国专利> METHOD FOR PREPARATION OF HEPATOCYTES WITH LIVER-SPECIFIC FUNCTIONS USING MESENCHYMAL STEM CELLS AND UMBILICAL CORD-DERIVED HEPATOCYTES PREPARED THEREBY

METHOD FOR PREPARATION OF HEPATOCYTES WITH LIVER-SPECIFIC FUNCTIONS USING MESENCHYMAL STEM CELLS AND UMBILICAL CORD-DERIVED HEPATOCYTES PREPARED THEREBY

机译:用间充质干细胞和脐带来源的肝细胞制备具有肝功能的肝细胞的方法

摘要

A method for manufacturing mesenchymal stem cell derived hepatocyte is provided to differentiate a mesenchymal stem cell which is derived from a wharton's jelly. A method for manufacturing mesenchymal stem cell derived hepatocyte comprises: a step of culturing the mesenchymal stem cell in cell proliferation medium; a step of inducing cells to hepatocyte in serum medium in which cell growth factor is added; and a step of maturing hepatocyte in serum medium in which a hepatocyte maturing induction material is added. The hepatocyte maturing induction material is selected from an oncostatin M, glucagon, nicotin amide and dimethyl sulfoxid(DMSO).
机译:提供了一种用于制造源自间充质干细胞的肝细胞的方法,以区分源自沃顿氏胶冻的间充质干细胞。制造间充质干细胞来源的肝细胞的方法包括:在细胞增殖培养基中培养间充质干细胞的步骤;在添加了细胞生长因子的血清培养基中诱导细胞到肝细胞的步骤;在血清培养基中使肝细胞成熟的步骤,其中添加了肝细胞成熟诱导材料。肝细胞成熟诱导材料选自制瘤素M,胰高血糖素,烟酰胺和二甲基亚砜(DMSO)。

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