首页> 外国专利> DEVELOPMENT OF A REAL TIME PCR FOR TOMATO YELLOW LEAF CURL SARDINIA VIRUS (TYLCSV) DETECTION AND QUANTIFICATION

DEVELOPMENT OF A REAL TIME PCR FOR TOMATO YELLOW LEAF CURL SARDINIA VIRUS (TYLCSV) DETECTION AND QUANTIFICATION

机译:番茄黄叶沙丁病毒(TYLCSV)检测和定量实时PCR的开发

摘要

Recently, tomato yellow leaf curl disease has become important for the tomato grown both in greenhouse and field conditions in Tunisia. Here, we describe a rapid, specific, reliable, and sensitive real-time PCR, based on TaqMan chemistry, for Tomato yellow leaf curl Sardinia virus (TYLCSV). This method proved suitable for the detection and quantification of this virus in tomato, pepper and bean plants. It detected the virus even in the samples that were negative by conventional assays.
机译:近来,番茄黄叶卷曲病对于突尼斯的温室和田间条件下生长的番茄已经变得重要。在这里,我们描述了一种基于TaqMan化学方法的快速,特异性,可靠和灵敏的实时PCR,用于番茄黄叶卷曲撒丁岛病毒(TYLCSV)。事实证明,该方法适用于番茄,胡椒和豆类植物中这种病毒的检测和定量。它甚至在常规检测为阴性的样品中也检测到了病毒。

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