首页>
外国专利>
A SIMPLE METHOD FOR ESTABLISHING OF IN VITRO REGENERATED PLANTS OF CHICKPEA IN SOIL'.
A SIMPLE METHOD FOR ESTABLISHING OF IN VITRO REGENERATED PLANTS OF CHICKPEA IN SOIL'.
展开▼
机译：建立土壤中鹰嘴豆离体再生植物的简单方法”。
展开▼
页面导航
摘要
著录项
相似文献
摘要
This invention relates to a simple and efficient method for establishing invitro regenerated plants of chick pea in soil by using plant growth promoting Rhizo bacteria PGPR, wherein the regenerated shoots are selected at a particular stage for rooting and regenerated shoots are first allowed root initiation, subsequent transfer to a particular medium; embryo axis explants were excised from presterilized, overnight soaked seeds, transferred to shooting medium containing MS basal with Bs vitamins and BAP O.lmg /I to 5.0 mg/1, preferably 2.0 mg/1, followed by transfer of regenerated shoots to a rooting medium MS basal with Bs vitamins and 1.0 mg/1 IBA along with 1.0 rng/1 NAA after 14 days, subsequently transfer the regenerated shoots where rooting has just initiated to a testube containing pre sterilized soil with a thin film of agar after 10 days, inoculating a loop full of PGPR culture near roots of plantlets.
展开▼
