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simple method of injectable chondrocyte transplantation for autologous chondrocyte transplantation
simple method of injectable chondrocyte transplantation for autologous chondrocyte transplantation
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机译：自体软骨细胞移植的简单注射软骨细胞移植方法
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摘要
Simple Method of Injectable Chondrocyte Transplantation for Autologous Chondrocyte Transplantation. It comprises mixing matrix containing fibrin, hyaluronic acid and collagen, which are the main ingredients of animal cartilage, by using a front mixing tip of a syringe, while the resulting mixture is injected into an injured cartilaginous region. In addition, the present invention provides a method of injectable chondrocyte transplantation for autologous chondrocyte transplantation, comprising the following steps: placing 1 ml of a chondrocyte culture contained in Vial No. 1 (Red) into a sterile 1-liter syringe. ml and inject the cell culture into Vial No. 2 (Blue) containing white or light yellow lyophilized powder (fibrinogen) by inserting the syringe into Vial No. 2 vertically so that the fibrinogen concentration varies between 10 mg / ml and 200 mg / ml; Inject 1 ml of the cell culture contained in Vial No. 1 (Red) into Vial No. 3 (Red, small) containing white lyophilized powder (thrombin) by inserting the syringe into Vial No. 3 vertically by injecting thus gradually the red liquid; Collect 0.1 cc of the 1 ml contents of Vial No. 3 (Red, small) using a 1 ml syringe and then inject into the bottom of Vial No. 4 (Yellow) such that the thrombin concentration ranges from 1 IU / mI to 200 IU / mI; Add the entire contents of two vials containing the chondrocyte suspension to Vial No. 4 (Yellow) using a 1 ml sterile syringe and then mix the contents (two or three times) using the syringe; Confirm complete dissolution of the contents of Vial No. 2 (Blue) and then inject all dissolved material into a 1 ml syringe; Inject the well-mixed Cell Therapeutic Agent from Bottle 4 (Yellow) into a 1 ml syringe, and; Mount two 1 ml syringes filled with the contents of Vial No. 2 (Blue) and Vial No. 4 (Yellow) into a fixed holder, then mount a front mixing tip to the syringes and mix the contents of the syringes. , through the use of the front mixing tip, while the resulting mixture to be transplanted is injected into an injured cartilaginous region of an animal. The present invention with the aforementioned construction offers advantageous effects, such as, for example, solution to the inconveniences presented by conventional osteoperiosteal graft by mixing and transplanting fibrin, hyaluronic acid and collagen containing matrices, which are the main ingredients of cartilage. ; treating a wide variety of cartilage defects and severe osteoarthritis; relieving the suffering of patients to be treated through a simplified method of surgical operation, and; consequently promoting faster and more effective cartilage generation, which results in better patient satisfaction.
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机译：用于自体软骨细胞移植的可注射软骨细胞移植的简单方法。它包括通过使用注射器的前端混合尖端将包含纤维蛋白，透明质酸和胶原蛋白（它们是动物软骨的主要成分）的混合基质，同时将所得混合物注入受伤的软骨区域。另外，本发明提供了用于自体软骨细胞移植的可注射软骨细胞移植的方法，其包括以下步骤：将1ml小瓶（红色）中包含的软骨细胞培养物1ml放入无菌的1升注射器中。通过将注射器垂直插入2号小瓶中，将细胞培养物注入包含白色或浅黄色冻干粉（纤维蛋白原）的2号小瓶（蓝色）中，使纤维蛋白原浓度在10 mg / ml和200 mg / ml之间变化;通过逐渐将红色液体逐渐注入到3号小瓶中，将注射器垂直插入3号小瓶中，将1 ml 1号小瓶（红色）中包含的细胞培养物注入含有白色冻干粉（凝血酶）的3号小瓶（红色，小）中。 ;使用1毫升注射器收集3毫升小瓶（1毫升）中的0.1毫升内容物，然后注入4毫升小瓶（黄色）的底部，使凝血酶浓度范围为1 IU / mI至200 IU / mI;用1 ml无菌注射器将两个装有软骨细胞悬液的小瓶的全部内容物加到第4号样品瓶（黄色）中，然后用注射器混合（两到三遍）内容物;确认2号样品瓶（蓝色）的内含物完全溶解，然后将所有溶解的材料注入1 ml注射器中;将来自第4瓶（黄色）的充分混合的细胞治疗剂注入1 ml注射器中;将装满2号样品瓶（蓝色）和4号样品瓶（黄色）的两个1 ml注射器安装到固定支架中，然后将前混合尖端安装到注射器上并混合注射器的内容物。通过使用前混合尖端，将要移植的所得混合物注射到动物受伤的软骨区域中。具有上述构造的本发明提供了有利的效果，例如，通过混合和移植作为软骨主要成分的纤维蛋白，透明质酸和含胶原的基质，解决了常规骨膜移植所带来的不便。 ;治疗各种软骨缺损和严重的骨关节炎;通过简化的手术方法减轻患者的痛苦，并且因此，促进了更快，更有效的软骨生成，从而提高了患者满意度。
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