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首页> 外国专利> METHOD FOR PRODUCING transplants containing beta cells of animal pancreas transplant patients with diabetes ANIMALS AND METHOD FOR THE TREATMENT OF DIABETES IN ANIMALS BY TRANSPLANTATION

METHOD FOR PRODUCING transplants containing beta cells of animal pancreas transplant patients with diabetes ANIMALS AND METHOD FOR THE TREATMENT OF DIABETES IN ANIMALS BY TRANSPLANTATION

机译：含有动物胰腺移植物的糖尿病动物的β细胞的移植物的制备方法及通过移植治疗糖尿病的方法

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1.method u043fu043eu043bu0443u0447u0435u043du0438u00a0 transplant u0434u043bu00a0 u043bu0435u0447u0435u043du0438u00a0 diabetes in dogs and cats, including the removal of the pancreas, its mechanical grinding processing u0434u0438u0441u043fu0435u0440u0433u0438u0440u0443u044eu0449u0438u043cu0438 solutions, re crushing, washing with saline u0445u0435u043du043au0441u0430 placement in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0443u044e environment and selection of cells u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 orderwhat u0434u043bu00a0 u0438u0437u0432u043bu0435u0447u0435u043du0438u00a0 pancreas using 262 days fruit rabbits or cats or dogs 563 days fruit, the re grinding of pancreatic p u0440u043eu0432u043eu0434u00a0u0442 to the size of 0.1 to 1.0 mm in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0443u044e breeding ground u0434u043eu0431u0430u0432u043bu00a0u044eu0442 5 - 10% u0444u0435u0442u0430u043bu044cu043du043eu0439 cow serum.and the selection of cells u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 by changing u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 environment every 3 - 4 u0434u043du00a0 on u043fu0440u043eu0442u00a0u0436u0435u043du0438u0438 10 - 14 days u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0, after which the cell clean out the nutritional environment and the serum in the u0445u0435u043du043au0441u0430 and placed in sterile saline solution.;2. method u043bu0435u0447u0435u043du0438u00a0 diabetes in dogs and cats, including transplant them - cells of pancreas, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 that u0434u043bu00a0 transplant use the graft obtained for 1, 5, 7 by injection in a dose of 0.2 ml u0432u0432u043eu0434u00a0u0442 dogs and cats directly through the abdominal wall in the left portion of the liver on u043fu0430u0440u0435u043du0445u0438u043cu0443 d control of ultrasonic u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0.
机译：1.犬和猫的糖尿病方法 u043f u043e u043b u0443 u0447 u0435 u043d u0438 u00a0移植 u0434 u043b u0040 u043b u0435去除胰腺，对其进行机械研磨处理 u0434 u0438 u0441 u043f u0435 u0440 u0433 u0438 u0440 u0443 u044e u0449 u0438 u043c u0438溶液，重新压碎，用盐水洗涤 u0445 u0435 u043d u043a u0441 u0430放置在 u043a u0443 u043b u044c u044c u0442 u0443 u0440 u0430 u043b u044c u043d u0443 u044e环境和单元格选择中 u043e u0442 u043b u0438 u0447 u0430 u044e u0449 u0438 u0439 u0441 u00a0顺序 u0434 u043b u00a0 u0438 u0437 u0432 u043b u0435 u0447 u0435 u043d u043d u0438 u00a0兔子使用或262天猫或狗563天的果实，将胰腺p u0440 u043e u0432 u043e u0434 u00a0 u0442重新研磨至0.1至1.0毫米，大小为 u043a u0443 u043b u044c u0442 u0443 u0440 u0430 u043b u044c u043d u0443 u044e繁殖地 u0434 u043e u0431 u0430 u04 32 u043b u00a0 u044e u0442 5-10％ u0444 u0435 u0442 u0430 u043b u044c u043d u043e u0439牛血清和细胞选择 u043e u0441 u0443 u0449 u0435 u3-41 u0442 u0432 u043b u00a0 u044e u0442通过每3-4 u043c u0443 u0440 u0430 u043b u044c u0443 u0440 u0430 u043b u043b u044c u043d u043d u043e u0439d更改环境 u043f u0440 u043e u0442 u00a0 u0436 u0435 u043d u0438 u0438 10-14天 u043a u0443 u043b u044c u0442 u0438 u0432 u0438 u0440 u043e u0432 u00a0然后清除细胞的营养环境和血清，并将其置于无菌盐溶液中; 2.。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。方法 u043b u0435 u0447 u0435 u043d u0438 u00a0糖尿病（包括将其移植）-胰腺， u043e u0442 u043b u0438 u0447 u0430 u044e u0449 u0438 u0439 u0434 u043b u00a0移植使用通过注射的0.2 ml剂量的1、5、7获得的移植物直接通过腹壁中的猫和狗 u043f u0430 u0440 u0435 u043d u0445 u0438 u043c u0443上肝脏的左侧部分d超声控制 u0438 u0441 u0441 u0431 u043b u0435 u0434 u043e u043e u0432 u0430 u043d u0438 u00a0。

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