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NOVEL PROBES, MULTIPLEX-PCR KIT, DNA CHIP AND PNA CHIP PREREQUISITE FOR HIGH-THROUGHPUT ANALYSIS AND SIMULTANEOUS ANTIBIOTICS RESISTANCE ANALYSIS TO DETECT INFECTIOUS DISEASES-RELATED MICROORGANISMS AND METHOD THEREOF
NOVEL PROBES, MULTIPLEX-PCR KIT, DNA CHIP AND PNA CHIP PREREQUISITE FOR HIGH-THROUGHPUT ANALYSIS AND SIMULTANEOUS ANTIBIOTICS RESISTANCE ANALYSIS TO DETECT INFECTIOUS DISEASES-RELATED MICROORGANISMS AND METHOD THEREOF
A method for diagnosis of infectious disease-related microorganisms is provided to detect the infection of infectious disease-related microorganisms and analyze their genotype and antibiotic resistance simultaneously through one-stop analysis, so that abuse of antibiotics is reduced and treatment period and expense are saved. A method for diagnosis of infectious disease-related microorganisms comprises the steps of: amplifying each disease group of the infectious disease-related microorganisms through multiplex-PCR(polymerase chain reaction) by using oligonucleotide primers of SEQ ID NOs:1 to 90; inducing hybridization of the PCR products with a probe corresponding to the disease, immobilized on a chip; and determining the hybridization, wherein the disease groups include urinary tract infection, rhinosinusitis, sexually transmitted disease, human papilloma virus, periodontitis and Mycobacteria infection, wherein the probe has an oligonucleotide selected from SEQ ID NOs:91-216.
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机译:本发明提供了一种诊断传染病相关微生物的方法,可以检测传染病相关微生物的感染并通过一站式分析同时分析其基因型和抗生素抗性,从而减少了抗生素的滥用,节省了治疗时间和费用。 。一种传染病相关微生物的诊断方法,包括以下步骤:使用SEQ ID NO:1至90的寡核苷酸引物通过多重PCR(聚合酶链反应)扩增该传染病相关微生物的每个疾病组。诱导PCR产物与固定在芯片上的对应于该疾病的探针的杂交;确定杂交,其中所述疾病组包括尿路感染,鼻-鼻窦炎,性传播疾病,人乳头瘤病毒,牙周炎和分枝杆菌感染,其中所述探针具有选自SEQ ID NO:91-216的寡核苷酸。
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