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METHOD AND DEVICE FOR RECONSTRUCTING A FLUORESCENCE OPTICAL TOMOGRAPHY THREE-DIMENSIONAL IMAGE BY DOUBLE MEASUREMENT
METHOD AND DEVICE FOR RECONSTRUCTING A FLUORESCENCE OPTICAL TOMOGRAPHY THREE-DIMENSIONAL IMAGE BY DOUBLE MEASUREMENT
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机译:通过二次测量重建荧光光学断层扫描三维图像的方法和装置
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摘要
To examine a plate-shaped object comprising fluorophores and having a first face and an opposite second face, the method comprises a first sequential illumination step of the first face of the object with a fluorophore excitation light and a first sequential acquisition step of a first series of images by detecting light emitted by the second face of the object. The density of the lighting points is lower than the density of the detection points and the method further comprises a second sequential illumination step of the second face of the object with a fluorophore excitation light and a second sequential acquisition step of a second series of images by detecting light emitted by the first face of the object. Reconstruction of the three-dimensional fluorophore distribution image in the object is performed by means of the first and second series of images.
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