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Proteome analysis method, proteome analysis system, and proteome analysis program

机译:蛋白质组分析方法,蛋白质组分析系统和蛋白质组分析程序

摘要

PPROBLEM TO BE SOLVED: To provide a method and a system that can reduce the number of experiments in identification with a small amount of a sample and an experiment for trial-and-error, adds a mass spectrum that may appear, a peak or the like buried in noise, or the like for achieving a high accuracy in protein identification and high throughput, and can determine sequencing using mass spectrometry even in new protein that is not registered in a protein sequencing database. PSOLUTION: In the mass spectrometry due to a tandem type mass spectrometry apparatus, bond dissociation energy is calculated by using a molecular simulation method, the prediction information of the cleavage pattern of peptide and/or the peak position of the mass spectrum and/or the intensity ratio of a spectrum is added to obtained experiment information for use as the identification of protein. PCOPYRIGHT: (C)2004,JPO
机译:

要解决的问题:提供一种方法和系统,可以减少少量样品的鉴定实验数量和反复试验的实验数量,并增加可能出现的质谱图。峰或类似物掩埋在噪音等中,以实现高精度的蛋白质鉴定和高通量,甚至可以使用质谱法确定测序,即使在未登录蛋白质测序数据库的新蛋白质中也是如此。

解决方案:在采用串联型质谱仪的质谱仪中,键解离能通过分子模拟方法,肽裂解模式的预测信息和/或质谱峰位置和/或将光谱的强度比添加到获得的实验信息中,以用作蛋白质鉴定。

版权:(C)2004,日本特许厅

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