首页> 外国专利> Method for isolating and purifying immunomodulatory polypeptides from bovine placenta

Method for isolating and purifying immunomodulatory polypeptides from bovine placenta

机译:从牛胎盘中分离和纯化免疫调节多肽的方法

摘要

The present invention provides a method for isolating and purifying immuno-modulating polypeptide from cow placenta, which is characterized by using the steps of anion-exchange chromatography, gel exclusion chromatography and reverse-phase high performance liquid chromatography to isolate and purify immuno-modulating polypeptide from cow placenta, identifying its activity of stimulating lymphocyte proliferation in vitro by MTT method, then determining its molecular weight by MALDI-TOF-MS, its isoelectric point by CIEF and its amino acid sequence with analyzer for protein sequencing. Since the obtained immuno-modulating polypeptide by the method according to the present invention has more than 90% purity, its bioactivity can reach medicinal standards.
机译:本发明提供了一种从牛胎盘中分离纯化免疫调节多肽的方法,其特征在于采用阴离子交换色谱法,凝胶排阻色谱法和反相高效液相色谱法分离纯化免疫调节多肽。用MTT法从牛胎盘中分离出其体外刺激淋巴细胞增殖的活性,然后用MALDI-TOF-MS测定其分子量,用CIEF测定其等电点,并用蛋白质分析仪测定其氨基酸序列。由于通过本发明的方法获得的免疫调节多肽具有超过90%的纯度,因此其生物活性可以达到医学标准。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号