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DISCRIMINATION METHOD OF JIN-DO NATIVE PLANTS PUNGRAN
DISCRIMINATION METHOD OF JIN-DO NATIVE PLANTS PUNGRAN
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机译：珍珍原生植物丰兰的鉴别方法
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摘要
The present invention relates to a method for discriminating native plant flora in Jindo.;The differentiation method of the jindosan native plant flanking of the present invention, Jindo-san gangsan DNA extraction step of extracting the DNA from the jindosan Fangran; A Jindo acid-like DNA amplification step of amplifying the extracted Jindo acid-like eggs by the RAPD-PCR assay using primers of SEQ ID NO: 1 or SEQ ID NO: 2; An electrophoresis of the amplified genomic genomic DNA of the jindo acid funnel, followed by a jindo acid funnel identification step for identifying a band position using an ultraviolet reader; A differential extraction DNA extraction step of preparing a differentiation target egg to be discriminated and extracting DNA of the differentiation object; A differentiating-target flanking DNA amplification step of amplifying the extracted differentiating DNA of flanking subjects using a primer of SEQ ID NO: 1 or SEQ ID NO: 2 by RAPD-PCR analysis; A step of identifying a differentiated flanking band for electrophoresis of genomic DNA of the amplified differentiated flies and confirming the band position using an ultraviolet reader; Comprising a discriminating step of discriminating the Jindo mountain wind turbulence by comparing the band position of the Jindo mountain wind turbulence and the band position of the wind turbine to discriminate.;According to the present invention, there is provided a method for easily discriminating the Jindo mountain turbulence that is difficult to discriminate with the naked eye, and a reproducible discrimination method for the Jindo mountain turbulence is provided.;Jindosan, Novel, Differentiation, Primer, SEQ ID NO: 1, SEQ ID NO: 2
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机译：本发明涉及一种鉴别金岛本地植物区系的方法。本发明的金岛山天然植物侧翼的分化方法，从金岛山方染中提取DNA的金岛山刚山DNA提取步骤; Jindo酸样DNA扩增步骤，其使用SEQ ID NO：1或SEQ ID NO：2的引物通过RAPD-PCR测定法扩增提取的Jindo酸样卵。对金杜酸漏斗的扩增的基因组基因组DNA进行电泳，然后进行金杜酸漏斗鉴定步骤，以使用紫外线读取器鉴定条带位置;差异提取DNA提取步骤，其制备待鉴别的分化目标卵并提取所述分化对象的DNA。分化目标侧翼DNA扩增步骤，通过SEQ ID NO：1或SEQ ID NO：2的引物通过RAPD-PCR分析来扩增提取的侧翼对象的分化DNA。鉴定用于扩增的分化的果蝇的基因组DNA的电泳的分化侧翼带并使用紫外线读取器确认带位置的步骤;通过比较金道山风湍的带位置和风力涡轮机的带位置来区分金道山风湍的判别步骤。根据本发明，提供了一种易于区分金岛风的方法。肉眼难以辨别的山间湍流，并提供了可重复的Jindo山间湍流判别方法.Jindosan，Novel，Novel，Differentiation，Primer，SEQ ID NO：1，SEQ ID NO：2
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