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ESTABLISHMENT OF THE ADIPOCYTE DIFFERENTIATION METHOD IN HANWOO INTRAMUSCULAR FIBROBLAST-LIKE CELLS AND ITS APPLICATION
ESTABLISHMENT OF THE ADIPOCYTE DIFFERENTIATION METHOD IN HANWOO INTRAMUSCULAR FIBROBLAST-LIKE CELLS AND ITS APPLICATION
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机译：汉窝核内成纤维细胞样细胞中脂肪细胞分化方法的建立及其应用
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摘要
A kind of method, it is shaped to excellent marbling in hanwoo settings by controlling nutrient in the supply of hanwoo for the Adipose Differentiation in the intramuscular fibroblast-like cells of hanwoo and its application, so as to material and the gene of Adipose Differentiation can be promoted to be screened in hanwoo. Method for the Adipose Differentiation in the intramuscular fibroblast-like cells of hanwoo includes the following steps:: the intramuscular fibroblast-like cells of hanwoo are isolated from hanwoo; The intramuscular fibroblast-like cells of hanwoo of isolation are cultivated in a medium, contain 33 muN biotins, 17 muM pantothenates, 1mM octanoic acids, 100 muM ascorbic acid, 10 mcg/ml insulin, 5 muM dexametasone, 10mM acetate and the 0. of 12-18 days3 isobutyl group -1-methylantine of 5mM.
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机译：通过控制韩宇肌中成纤维样细胞内脂肪分化的营养供应和其应用，可以使其在韩宇环境中具有良好的大理石花纹，从而形成脂肪分化的材料和基因。晋升为在韩宇放映。汉宇肌内成纤维样细胞的脂肪分化方法包括以下步骤：从汉宇分离出汉宇肌内成纤维样细胞。在培养基中培养分离出的hanwoo的肌内成纤维细胞样细胞，该细胞包含33μN生物素，17μM泛酸，1mM辛酸，100μM抗坏血酸，10 mcg / ml胰岛素，5μM地塞米松，10mM乙酸盐和0。 12-18天3的异丁基-1-甲嘌呤为5mM。
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