首页>
外国专利>
A NOVEL PROCESS FOR SUSTAINABLE PRODUCTION OF BAMBOO SEEDS THROUGH COMBINED IN VITRO FLOWERING AND VITRO SEEDING
A NOVEL PROCESS FOR SUSTAINABLE PRODUCTION OF BAMBOO SEEDS THROUGH COMBINED IN VITRO FLOWERING AND VITRO SEEDING
展开▼
机译：竹种子体外育种和体外播种相结合的可持续生产新工艺。
展开▼
页面导航
摘要
著录项
相似文献
摘要
1. A novel process for the sustainable production of Bambusa bambos (thorny bamboo) flowers and seeds through a combined process of in vitro flowering and ex vitro seeding comprising the steps of cold treatment and storage of the seeds by collecting the mature seeds, sundrying the said seeds at 27-30°C for a period of 30 minutes and tightly packing the dried seeds in polythene bags for a minimum period of 6 weeks at 0°C temperature in a conventional freezer; germinating the said treated seeds by thawing and manually de-husking the seeds; thoroughly washing the de-husked seeds in 2% (v/v) Teepol solution for 15 minutes followed by continuous washing in running tap water for 1 hr and surface sterilizing the said seeds in 4% sodium hypochlorite solution for 15-20 minutes followed by passage through 0.1 % (w/v) HgCI2 for 7 minutes; washing the seeds 4-5 times in sterile distilled water and inoculating the said seeds into 15 ml of sterile nutrient media in 15x250 mm culture tubes; culturing the seeds as claimed in the preceding claims on to Murashige and Skoog, 1962 (MS) agar nutrient medium (0.6% agar, pH 5.8) supplemented with 3% (w/v) sucrose; germinating the said seeds in presence of 0.764pM BAP and 1.44pM GA3 to produce multiple shoots in four weeks in the culture room maintained at temperatures between 23°C to 25°C under 8 hr photoperiod to enable the shoots to set flowers in 6-8 weeks( after seed sowing in the nutrient medium); separation of the shoots with flowers by subculturing the same for a 4 week period in the same MS nutrient medium in 60 ml aliquots in 250 ml Erlenmeyer conical flasks in the presence of the same hormonal regime (0.76pM BAP and 1.44pM GA3) for multiplication of 100% flowering shoots; successive sub-culturing
展开▼
