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Procedure For The Fractionation Of Proteins By Using Sequential Ion Exchange And Hydrophobic Interaction Chromatography As Prefractionation Steps Before Analysis By Two Dimensional Electrophoresis
Procedure For The Fractionation Of Proteins By Using Sequential Ion Exchange And Hydrophobic Interaction Chromatography As Prefractionation Steps Before Analysis By Two Dimensional Electrophoresis
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机译:二维电泳分析之前,使用顺序离子交换和疏水相互作用色谱作为预分离步骤进行蛋白分离的步骤
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摘要
After the sequencing of the human genome, great interest has developed in trying to discern the complementary proteome of humans and other species. The present disclosure provides devices, systems, and methods for proteomic fractionation that may increase the number of protein spots visualized by 2DE analysis, and may allow enrichment of proteins normally not detectable by standard 2DE analysis. According to some embodiments of the disclosure, devices, systems, and methods of the disclosure relate to fractionating a proteome on the basis of surface charge, hydrophobicity, isoelectric point and/or size.
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