首页> 外国专利> RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 pREP4, pLACspCBD AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE

RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 pREP4, pLACspCBD AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE

机译：重组蛋白LacspCBD具有β-半乳糖苷酶的活性和与含纤维素的吸附剂自发结合的能力，重组质粒DNA编码的重组蛋白LacspCBD，重组大肠杆菌蛋白酶解粘蛋白蛋白酶解蛋白4，其蛋白酶解蛋白蛋白酶解酶原蛋白酶[4]。纤维素上的蛋白LacspCBD和酶切乳糖的方法

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FIELD: genetic engineering, biotechnology.;SUBSTANCE: invention proposes a fused protein of beta-galactosidase and cellulose-binding domain of endoglucanase CelD from Anaerocellum thermophilium. The recombinant plasmid DNA pLACspCBD is constructed providing its expression in E. coli cells. The strain E. coli as a producer of indicated fused protein is obtained. A method for preparing this fused protein in immobilized form is developed that involves treatment of cellulose with hydrolyzate of the indicated strain of E. coli. Using the invention provides simplifying processing of dairy foodstuffs. Invention can be used in food processing industry.;EFFECT: valuable properties of strain and protein, improved preparing method.;5 cl, 1 dwg, 5 ex

机译：领域：本发明提出了一种嗜热厌氧厌氧杆菌的β-半乳糖苷酶和内切葡聚糖酶CelD的纤维素结合域的融合蛋白。构建重组质粒DNA pLACspCBD，使其在大肠杆菌细胞中表达。获得作为指定融合蛋白生产者的大肠杆菌菌株。开发了一种制备固定形式的这种融合蛋白的方法，该方法包括用所示大肠杆菌菌株的水解产物处理纤维素。使用本发明可简化乳制品的加工。发明效果可用于食品加工业。效果：菌株和蛋白质的宝贵特性，改进的制备方法。5 cl，1 dwg，5 ex

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公开/公告号RU2278160C2

专利类型
公开/公告日2006-06-20

原文格式PDF
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申请/专利号RU20040110981
发明设计人 VELIKODVORSKAJA GALINA ALEKSANDROVNA;ZVERLOV VLADIMIMR VLADIMIROVICH;KARJAGINA-ZHULINA ANNA STANISLAVOVNA;LAVROVA NATALJA VITALEVNA;LUNIN VLADIMIR GLEBOVICH;LUNINA NATALIJA ALEKSANDROVNA;RJAZANOVA ELENA MIKHAJLOVNA;SERGIENKO OLGA VASILEVNA;TIKHONOVA TATJANA VJACHESLAVOVNA;
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申请日2004-04-13
分类号C12N9/38;C12N15/56;C12N15/72;C12N1/21;C12N11/12;C12P19;C12R1/19;
国家 RU
入库时间 2022-08-21 21:21:35

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