• 主题
高级搜索  >
历史搜索 清空历史
首页> 外国专利> RECOMBINANT PLASMID DNA ENCODING TUL4spCBD RECOMBINANT PROTEIN SYNTHESIS, M15pREP4, pTUL4spCBD ESCHERICHIA COLI STRAIN AS PRODUCER OF TUL4spCBD RECOMBINANT PROTEIN, TUL4spCBD RECOMBINANT PROTEIN, METHOD FOR PRODUCTION THEREOF, AND METHOD FOR PRODUCTION OF SPECIFIC ANTIBODY AGAINST TUL4spCBD PROTEIN

RECOMBINANT PLASMID DNA ENCODING TUL4spCBD RECOMBINANT PROTEIN SYNTHESIS, M15pREP4, pTUL4spCBD ESCHERICHIA COLI STRAIN AS PRODUCER OF TUL4spCBD RECOMBINANT PROTEIN, TUL4spCBD RECOMBINANT PROTEIN, METHOD FOR PRODUCTION THEREOF, AND METHOD FOR PRODUCTION OF SPECIFIC ANTIBODY AGAINST TUL4spCBD PROTEIN

机译：包含Tul4spCBD重组蛋白合成的重组质粒DNA，M15 [pREP4，pTUL4spCBD]大肠杆菌菌株，作为Tul4spCBD重组蛋白的生产者，Tul4spCBD重组蛋白的生产方法，产物的制备方法和方法

页面导航
  • 摘要
  • 著录项
  • 相似文献

摘要

FIELD: biotechnology, gene engineering, microbiology.;SUBSTANCE: invention relates to TUL4spCBD recombinant protein comprising TUL4 Francisella tulergenis protein, Gly-Ser spacer and cellulose-binding domain of CelD endoglucanase gen from Anaerocellum thermophilus. Said protein has TUL protein antigen properties and is capable of spontaneous binding to cellulose-containing sorbents. Described is recombinant plasmid pTUL4spCBD DNA of 4388 n.p. length, encoding TUL4spCBD recombinant protein. Abovementioned plasmid contains: artificial bacterial operon of TUL4spCBD recombinant protein, including promoter region of N5 bacteriophage earlier promoter (7-87 n.p.); TUL4spCBD recombinant protein gene (115-1113 n.p.); transcription terminator (1134-1230 n.p.); beta-lactamase bacterial operon (4183-3323 n.p. of complementary chain)), providing ampicillin resistance; ColE1-type bacterial site of replication initiation, providing plasmid replication in E.coli strain (4388 n.p.). Also discoised is M15[pREP4, pTUL4spCBD] Escherichia coli strain as producer of TUL4spCBD recombinant protein and method for production of purified, concentrated and immobilized TUL4spCBD recombinant protein from said strain by treatment of cell hydrolyzate supernatant from M15[pREP4, pTUL4spCBD] Escherichia coli with cellulose-containing sorbent. Method for production of specific antibodies against TUL4spCBD protein also is described. Said method includes animal immunization with TUL4spCBD recombinant protein immobilized onto cellulose.;EFFECT: method for high yield production of TUL4spCBD recombinant protein; simplified method for purification, concentration and immobilization of said protein.;5 cl, 1 dwg, 4 ex
机译：技术领域：本发明涉及TUL4spCBD重组蛋白，其包含来自嗜热厌氧杆菌的TUL4弗朗西斯菌Tulergenis蛋白，Gly-Ser间隔子和CelD内切葡聚糖酶基因的纤维素结合结构域。所述蛋白质具有TUL蛋白质抗原特性，并且能够自发结合至含纤维素的吸附剂。描述了4388n.p的重组质粒pTUL4spCBD DNA。长度，编码TUL4spCBD重组蛋白。上述质粒包含：TUL4spCBD重组蛋白的人工细菌操纵子，包括N5噬菌体早期启动子的启动子区域（7-87n.p。）; TUL4spCBD重组蛋白基因（115-1113 n.p.）;转录终止子（1134-1230 n.p.）; β-内酰胺酶细菌操纵子（4183-3323 n.p.，互补链）），具有氨苄青霉素抗性; ColE1型细菌复制起点，可在大肠杆菌菌株（4388 n.p.）中提供质粒复制。还公开了作为TUL4spCBD重组蛋白的生产者的M15 [pREP4，pTUL4spCBD]大肠杆菌菌株，以及通过用M15 [pREP4，pTUL4spCBD]大肠杆菌的细胞水解上清液处理来自该菌株的纯化，浓缩和固定化的TUL4spCBD重组蛋白的方法。含纤维素的吸附剂。还描述了产生针对TUL4spCBD蛋白的特异性抗体的方法。所述方法包括用固定在纤维素上的TUL4spCBD重组蛋白进行动物免疫。纯化，浓缩和固定所述蛋白的简便方法。; 5 cl，1 dwg，4 ex

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号