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FLUORESCENCE POLARIZATION-BASED METHOD FOR ANALYSIS OF FARNESOID X RECEPTOR-LIGAND COMPLEX INTERACTION AND FOR SCREENING INHIBITORS AGAINST THE FARNESOID X RECEPTOR-LIGAND COMPLEX BINDING
FLUORESCENCE POLARIZATION-BASED METHOD FOR ANALYSIS OF FARNESOID X RECEPTOR-LIGAND COMPLEX INTERACTION AND FOR SCREENING INHIBITORS AGAINST THE FARNESOID X RECEPTOR-LIGAND COMPLEX BINDING
The present invention relates to a method for analyzing the interaction of a panesoid X receptor-ligand complex using fluorescence polarization and a method for screening binding inhibitors, and specifically, between a panesoid X receptor (FXR) and a fluorescently labeled ligand using fluorescence polarization. Interaction analysis method and binding inhibitory candidates were added to samples containing fluorescently labeled ligands and Panesoid X receptors, and fluorescent fluorescence was measured to confirm competitive binding of ligands to inhibitors of binding to Panesoid X receptors. The present invention relates to a method for screening a binding inhibitor of a panesoid X receptor and a ligand complex. The interaction analysis method and binding inhibitor search method of the present invention can be effectively applied to the ultra-fast search using the well plate is very useful for the development of hyperlipidemia therapeutics.;Panesoid X Receptor, Fluorescence Polarization, Ligand
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