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ANTIBODY-PRODUCING CELL LINES EXPRESSING ACTIVATION-INDUCED-CYTIDINE-DEAMINASE AND A DOMINANT NEGATIVE ALLELE OF A MISMATCH REPAIR GENE
ANTIBODY-PRODUCING CELL LINES EXPRESSING ACTIVATION-INDUCED-CYTIDINE-DEAMINASE AND A DOMINANT NEGATIVE ALLELE OF A MISMATCH REPAIR GENE
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机译:表达失活修复基因的激活诱导的胞嘧啶脱氨酶和显性负等位基因的抗体生产细胞系
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摘要
Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. Cells may be selected for expression of activation-induced cytidine deaminase (AID), stimulated to produce AID, or manipulated to express AID for further enhancement of hypermutability. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production.
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