首页> 外国专利> NUCLEOTIDE SEQUENCES AND METHODS FOR THE SPECIFIC EXPRESSION OF GENES IN THE FEMALE GAMETOPHYTE, FEMALE REPRODUCTIVE CELLS, POLLEN GRAIN AND/OR MALE REPRODUCTIVE CELLS OF PLANTS

NUCLEOTIDE SEQUENCES AND METHODS FOR THE SPECIFIC EXPRESSION OF GENES IN THE FEMALE GAMETOPHYTE, FEMALE REPRODUCTIVE CELLS, POLLEN GRAIN AND/OR MALE REPRODUCTIVE CELLS OF PLANTS

机译:在雌性游戏植物,雌性生殖细胞,花粉粒和/或雄性生殖细胞中基因表达的核苷酸序列和方法

摘要

The invention relates to the localisation and expression patterns of promoters (regulatory nucleotide sequences) which act in a specific manner in the female gametophyte, the female gametes, the male gametophyte and/or the male gametes of Arabidopsis thaliana. The invention also relates to the methods that were used to identify the aforementioned promoters as well as the methods of using said promoters in expression constructions and to manipulate the reproductive development of plants. According to the invention, the identified, isolated promoters can be used to regulate the genic expression of selected genes in plants, so that such genes can be efficiently transcribed in the female or male reproductive cells of same. Said promoters can be used to prevent the transfer of transgenic traits to plants through in-field pollen in order to provide the seed with additional nutrients and to confer resistance to pathogens and diseases to the seed.
机译:本发明涉及在拟南芥的雌配子体,雌配子,雄配子体和/或雄配子中以特定方式起作用的启动子(调节性核苷酸序列)的定位和表达模式。本发明还涉及用于鉴定上述启动子的方法,以及在表达构建中使用所述启动子和操纵植物生殖发育的方法。根据本发明,所鉴定的分离的启动子可以用于调节植物中所选基因的基因表达,从而可以在相同的雌性或雄性生殖细胞中有效地转录此类基因。所述启动子可用于防止转基因性状通过田间花粉转移至植物,以便为种子提供额外的营养,并赋予种子对病原体和疾病的抗性。

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