首页> 外国专利> TRANSFORMED LEPIDOPTERAN CELL LINES THAT EXPRESS HIIGH LEVELS OF FUNCTIONAL MAMMALIAN OPIOID RECEPTORS AS SCREENING SYSTEMS FOR THE DETECTION OF OPIOID AGONISTS AND ANTAGONISTS

TRANSFORMED LEPIDOPTERAN CELL LINES THAT EXPRESS HIIGH LEVELS OF FUNCTIONAL MAMMALIAN OPIOID RECEPTORS AS SCREENING SYSTEMS FOR THE DETECTION OF OPIOID AGONISTS AND ANTAGONISTS

机译：表达功能性哺乳动物阿片受体高水平的转化的蛛网膜细胞系，作为检测阿片类药物和拮抗剂的筛选系统

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摘要

Described herein are tools and a method for identifying oioid ligands (agonists or antagonists) that bind and (in)activate mammalian Opiod reseptors. The method is based on the use of stably transformed lepidopteeran cell lines that over-express mammalian opioid receptors. In some cell lines, the promiscuous Caq protein, Ga16, is also over-expressed to enhance the coupling of the over-expressed opioid receptors to the Ca2= signaling pathway. Opioid activity is monitored through the measurementsof intrecellular Ca2= release by fluorescent or luminescent methods.

机译：本文描述了用于鉴定与哺乳动物的Opiod受体结合和（激活）卵磷脂配体（激动剂或拮抗剂）的工具和方法。该方法基于使用过度表达哺乳动物阿片受体的稳定转化的鳞翅类细胞系。在某些细胞系中，混杂的Caq蛋白Ga16也被过度表达，以增强过度表达的阿片受体与Ca2 =信号通路的偶联。阿片样物质的活性通过荧光或发光方法测量细胞内Ca2 =释放来监测。

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公开/公告号GR1005105B

专利类型
公开/公告日2006-01-13

原文格式PDF
申请/专利权人 IATROU KONSTANTINOS;SWEVERS LUC;GEORGOUSI ZAFEIROULA;ETHNIKO KENTRO EREVNAS FYSIKON EPISTIMON DIMOKRITOS;
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申请/专利号GR20040100397
发明设计人 SWEVERS LUC;GEORGOUSI ZAFEIROULA;IATROU KONSTANTINOS;
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申请日2004-10-07
分类号C07K14/705;C12N5/00;C12N15/63;C12N15/866;
国家 GR
入库时间 2022-08-21 21:39:32

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