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Gene expression of alpha and beta chains of hormu00c8nio folu00ecculo stimulating (FSH) of Bos taurus indicus in yeast and cultured cells of mamu00ecferos
Gene expression of alpha and beta chains of hormu00c8nio folu00ecculo stimulating (FSH) of Bos taurus indicus in yeast and cultured cells of mamu00ecferos
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机译：牛和牛的酵母和培养细胞中的荷尔蒙刺激（FSH）的α和β链的基因表达
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摘要
"Gene expression of alpha and beta of the chairs hormu00c8nio folu00ecculo stimulating (FSH) of Bos taurus indicus in yeast and cultured cells of mamu00ecferos".For the preparation of hormu00f3nio expression of follicle stimulating (FSH) of Bos taurus indicus in yeast and mammalian cells have been carried out constructions that allow the production of this hormu00f3nioBicistru00f3nico separately with tape and as a fusion protein with the genes of the alpha chain and beta fused.These buildings have also been developed with 100% preference codons (codon usage) for yeasts and also containing 47% by GC.As for the expression in mammalian cells, the codons were not changed, and, therefore, the original gene sequences, not yet described in the literature, national and international.However, they were merged to be in the form of fusion protein.The product of these constructions, which is the FSH is used for processes of embryo transfer, in vitro fertilization and and treatment of infertility of Bos taurus indicus.
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