首页>
外国专利>
Cryopreservation method of stem cells derived from human blastocysts using the closed straw vitrification method
Cryopreservation method of stem cells derived from human blastocysts using the closed straw vitrification method
展开▼
机译：封闭秸秆玻璃化法冷冻保存人胚泡干细胞的冷冻方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
An improved method for vitrification of biological cells, especially blastocyst-derived stem cells (BS cells). The method is very mild for the cells that remain viable after they have been thawed. The method comprises, i) transfer of the cells to a first solution (solution A), ii) optionally incubation of the cells in the first solution, iii) transfer the cells obtained in step i) or ii) to a second solution (solution B), iv) optionally incubation of the cells in the second solution, v) transfer of the cells obtained from step iii) or iv) into one or more closed straws with dimensions that allow a volume of at least 20 žl to be contained in them vi) sealing the one or more closed straws, and vii) vitrification of the one or more closed straws. An important feature of the present invention is the use of closed straw and that relatively large volumes can be efficiently vitrified and subsequently thawed.
展开▼
