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首页> 外国专利> A method for the production of l - amino acids by means of fermentation of a escherichia strain transformed with a pyruvate - carboxyl lase gene from bacillus subtilis

A method for the production of l - amino acids by means of fermentation of a escherichia strain transformed with a pyruvate - carboxyl lase gene from bacillus subtilis

机译：一种通过发酵枯草芽孢杆菌的丙酮酸-羧化酶基因转化的大肠埃希氏菌菌株生产l-氨基酸的方法

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FIELD: biotechnology, microbiology, genetic engineering, biochemistry, amino acids. SUBSTANCE: method involves to preparing L-amino acids as such L-threonine, L-homoserine, L-methionine, L-arginine, L- proline, L-isoleucine or L-glutamic acid by culturing the microorganism Escherichia coli transformed with gene encoding enzyme pyruvate carboxylase being this strain E. coli shows ability to produce L-amino acids. Accumulated L-amino acids are extracted from nutrient medium. The strain of bacterium E. coli MG 442 or 44 is transformed with plasmid comprising the gene encoding the enzyme pyruvate carboxylase. By the first variant the strain produces L-threonine or L-glutamic acid and by the latter variant L-homoserine. Invention provides the high effectiveness of production of L-amino acids. EFFECT: improved preparing method, valuable properties of strain. 5 cl, 3 dwg, 1 tbl, 3 ex
机译：领域：生物技术，微生物学，基因工程，生物化学，氨基酸。物质：该方法涉及通过培养转化有编码基因的大肠杆菌来制备L-氨基酸，例如L-苏氨酸，L-高丝氨酸，L-蛋氨酸，L-精氨酸，L-脯氨酸，L-异亮氨酸或L-谷氨酸丙酮酸羧化酶是该菌株，大肠杆菌显示出产生L-氨基酸的能力。从营养培养基中提取积累的L-氨基酸。用包含编码丙酮酸羧化酶的基因的质粒转化大肠杆菌MG 442或MG 44的菌株。通过第一个变体，菌株产生L-苏氨酸或L-谷氨酸，而通过后一个变体，产生L-高丝氨酸。本发明提供了生产L-氨基酸的高效率。效果：改进的制备方法，有价值的菌株特性。 5 cl，3 dwg，1 tbl，3 ex

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