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Fermentative preparation of L-lysine-containing product by inoculating and culturing coryneform bacteria in nutrient medium, continuously supplying nutrient medium to culture for producing L-lysine, removing culture broth from culture
Fermentative preparation of L-lysine-containing product by inoculating and culturing coryneform bacteria in nutrient medium, continuously supplying nutrient medium to culture for producing L-lysine, removing culture broth from culture
Fermentative preparation (M1) of an L-lysine-containing product using coryneform bacteria which produces L-lysine, involves inoculating and culturing the bacterium in a first nutrient medium, continuously supplying nutrient medium or media to the culture, where the nutrient medium comprises carbon, nitrogen and phosphorus source, and removing the culture broth from the culture. Fermentative preparation (M1) of an L-lysine-containing product using coryneform bacteria which produces L-lysine, involves inoculating and culturing the bacterium in a first nutrient medium, continuously supplying nutrient medium or media to the culture in one feed stream (F1) or several feed streams (F1+), under conditions which allow the formation of L-lysine, where (a) the nutrient medium or media comprises one or more source of carbon (not more than 10 g/l), nitrogen and phosphorus, (b) the cells are completely or partly separated off from the removal stream (F4) with a return flow ratio of 0 less than R=1 by appropriate processes and are recycled into the culturing batch (F5), and (c) the formation of L-lysine, based on lysine HCl, takes place with an output index (OI) [OI=space/time yield (g/(lXh))Xyield(w/w)Xconcentration of lysine HC1 (g/l)] of 130 g2/(l2Xh) or more, and removing the culture broth from the culture with a removal stream (F2) or several removal streams (F2+) which substantially corresponds/correspond to the feed stream (F1) or the sum of the feed streams (F1+).
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