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THE THAWING CONNECTIVE INSTRUMENT AND THAWING METHOD FOR CRYOPRESERVED UMBILICAL CORD BLOOD CELLS USING THE THAWING CONNECTIVE INSTRUMENT
THE THAWING CONNECTIVE INSTRUMENT AND THAWING METHOD FOR CRYOPRESERVED UMBILICAL CORD BLOOD CELLS USING THE THAWING CONNECTIVE INSTRUMENT
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机译:利用解冻连接工具解冻冷冻脐带血细胞的解冻连接工具和解冻方法
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摘要
The present invention removes DMSO, a toxic component added to increase umbilical cord blood cell cryopreservation during cryopreservation, and maximizes the recovery of viable cells during thawing to thaw cryopreserved umbilical cord blood cells to increase the likelihood of successful umbilical cord blood cell transplantation. It relates to a connection mechanism and a thawing method using the same.;Specifically, thawing cord blood cells frozen in the freezing bag using a thawing solution consisting of 5% Dextran-40 + 5% albumin formed by mixing 10% Dextran-40 and 20% human serum albumin, It is characterized by providing a connection mechanism for thawing that is installed to supply.;In addition, it is characterized by providing a method for thawing cord blood cells using the thawing solution and the thawing coupling mechanism.;Accordingly, by thawing under the closed-circuit system as described above, cryopreserved umbilical cord blood cells can be thawed aseptically to recover more nucleated cells more stably, and show higher cell viability. To make it visible.
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