首页> 外国专利> THE THAWING CONNECTIVE INSTRUMENT AND THAWING METHOD FOR CRYOPRESERVED UMBILICAL CORD BLOOD CELLS USING THE THAWING CONNECTIVE INSTRUMENT

THE THAWING CONNECTIVE INSTRUMENT AND THAWING METHOD FOR CRYOPRESERVED UMBILICAL CORD BLOOD CELLS USING THE THAWING CONNECTIVE INSTRUMENT

机译:利用解冻连接工具解冻冷冻脐带血细胞的解冻连接工具和解冻方法

摘要

The present invention removes DMSO, a toxic component added to increase umbilical cord blood cell cryopreservation during cryopreservation, and maximizes the recovery of viable cells during thawing to thaw cryopreserved umbilical cord blood cells to increase the likelihood of successful umbilical cord blood cell transplantation. It relates to a connection mechanism and a thawing method using the same.;Specifically, thawing cord blood cells frozen in the freezing bag using a thawing solution consisting of 5% Dextran-40 + 5% albumin formed by mixing 10% Dextran-40 and 20% human serum albumin, It is characterized by providing a connection mechanism for thawing that is installed to supply.;In addition, it is characterized by providing a method for thawing cord blood cells using the thawing solution and the thawing coupling mechanism.;Accordingly, by thawing under the closed-circuit system as described above, cryopreserved umbilical cord blood cells can be thawed aseptically to recover more nucleated cells more stably, and show higher cell viability. To make it visible.
机译:本发明去除了DMSO,DMSO是在冷冻保存期间增加以增加脐带血细胞冷冻保存的有毒成分,并且在解冻以冷冻保存的脐带血细胞的过程中使活细胞的回收最大化,从而增加了成功脐带血细胞移植的可能性。本发明涉及一种连接机制和使用该融解方法的解冻方法。具体地,使用由5%Dextran-40 + 5%白蛋白组成的解冻溶液融化在冷冻袋中冷冻的脐带血细胞,该解冻溶液由10%Dextran-40和20%人血清白蛋白,其特征在于提供一种用于安装的解冻连接机制。此外,其特征在于提供一种使用解冻溶液和解冻偶联机制解冻脐带血细胞的方法。通过在如上所述的闭路系统下解冻,可以将冷冻保存的脐带血细胞无菌地融化,从而更稳定地回收更多的有核细胞,并显示出更高的细胞生存力。使它可见。

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