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GENE CLONING FOR THE NOVEL GLUTATHIONE S-TRANSFERASE ISOLATED FROM ORYZA SATIVA AND METHOD FOR MASS-PRODUCING THE SAME RECOMBINANT PROTEIN
GENE CLONING FOR THE NOVEL GLUTATHIONE S-TRANSFERASE ISOLATED FROM ORYZA SATIVA AND METHOD FOR MASS-PRODUCING THE SAME RECOMBINANT PROTEIN
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机译：谷胱甘肽分离的新型谷胱甘肽S-转移酶的基因克隆及其大规模生产相同重组蛋白的方法
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摘要
The invention process and this means that protein, Os GSTF3-3 (glutatione S -transferase), which is expressed by angling for the existing other new gene and the known sequence of AF309384 Rice derived by default through a gene cloned as disclosed in Gene Bank and mass-produced by (a) molecular weight 24kDa the protein; (B) Optimum pH 9.0; (C) Optimum temperature 45 ℃; And in (d) CDNB (1-chloro -2,4-dinitrobenzene), ETA (ethacrynic acid), EPNP (1,2-epoxy-3- (p -nitrophenoxy) propane) and Acetanilde herbicides (alachlor, metolachlor) for recombinant expression containing the new recombinant GST protein and gene coding for the protein derived from rice, it characterized in that it has the characteristics of the substrate specificity vector using the pET- Os GSTF3 relates to a method for mass production of proteins.; Recombinant GST protein in the rice-derived mass-produced in E. coli according to the method of the present invention may be useful in plant cells detoxification metabolism analysis caused by herbicide, pesticide or substance. Further, as a target molecule or a tumor marker (cancer marker) it can be usefully employed in a variety of fields that need thereof a diagnostic and chemotherapeutic field.
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机译：本发明的过程意味着蛋白质 Os GSTF3-3（谷氨酸 S -转移酶）是通过钓鱼现有的其他新基因和已知序列来表达的。 AF309384水稻默认情况下是通过基因库中公开的基因克隆而获得的，并通过（a）分子量为24kDa的蛋白质进行大量生产; （B）最适pH 9.0; （C）最佳温度45℃;在（d）CDNB（1-氯-2,4-二硝基苯），ETA（乙炔酸），EPNP（1,2-环氧-3-（对硝基苯氧基）丙烷）和乙腈除草剂（丙草胺，甲草胺）中含有新的重组GST蛋白和编码该蛋白的基因的重组表达，其特征在于它具有利用pET- Os GSTF3作为底物特异性载体的特征，涉及一种批量生产方法蛋白质的产生。根据本发明的方法，在大肠杆菌中生产的大米衍生的大规模生产中的重组GST蛋白可用于由除草剂，农药或物质引起的植物细胞解毒代谢分析。此外，作为靶分子或肿瘤标志物（癌症标志物），其可有效地用于需要其诊断和化学治疗领域的各种领域。
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