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首页> 外文会议>Annual Meeting of the Plant Growth Regulation Society of America >MOLECULAR CLONING AND ANALYSIS OF ORYZA SATIVA AUXIN-BINGDING PROTEIN 1 GENE
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MOLECULAR CLONING AND ANALYSIS OF ORYZA SATIVA AUXIN-BINGDING PROTEIN 1 GENE

机译：Oryza Sativa毒素轰炸蛋白1基因的分子克隆与分析

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摘要

In order to study Oryza sativa auxin-binding protein 1, the authors analyzed and obtained its full-length cDNA sequence by bioinformatics and laboratory bench-work. Firstly, an unnamed Oryza sativa cDNA sequence was obtained from nucleotide sequence database of GenBank, the putative open reading frame of the sequence encodes a presumptive protein containing 206 ammo acid residues. Blast against the current GenBank DNA and protein sequence database reveal significant homology with the ABP1 of other plants, and the presumptive conformation may resemble these of the ABP1 of other plants. These results suggest the sequence is Oryza sativa ABP1 cDNA. Based the sequence, ABP1 cDNA of Oryza sativa was amplified with RT-PCR and cloned for sequencing, the result of sequencing accord with electronic search result.
机译：为了研究Oryza Sativa植物蛋白结合蛋白1，作者通过生物信息学和实验室工作台进行了分析并获得了其全长cDNA序列。首先，从Genbank的核苷酸序列数据库获得未命名的ORYZA苜蓿cDNA序列，该序列的推定开放阅读框编码含有206个氨酸残基的推定蛋白质。对目前的Genbank DNA和蛋白质序列数据库的爆炸揭示了其他植物的ABP1的显着同源性，并且推测构象可以类似于其他植物的ABP1。这些结果表明序列是Oryza Sativa ABP1 cDNA。基于该序列，用RT-PCR扩增Oryza Sativa的ABP1 cDNA，并克隆用于测序，符合电子搜索结果的结果。

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