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FLOW-THROUGH SHEAR ANALYZER FOR BIOLOGICALLY ACTIVE MOLECULES IN LIQUID LAYERS ON SURFACES, METHOD OF ANALYSING A LIQUID AND METHOD OF DETERMINING THE THICKNESS OF AN ULTRA THICK LIQUID LAYER
FLOW-THROUGH SHEAR ANALYZER FOR BIOLOGICALLY ACTIVE MOLECULES IN LIQUID LAYERS ON SURFACES, METHOD OF ANALYSING A LIQUID AND METHOD OF DETERMINING THE THICKNESS OF AN ULTRA THICK LIQUID LAYER
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机译：表面液体层中生物活性分子的流变剪切分析仪，液体分析方法和超厚液体层厚度的测定方法
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摘要
The adsorption rate of proteins from solutions on surfaces in the region of interface layers is often so large that a depletion of the protein in the interface layer results. Due to this, the total reaction becomes transport-dependent, sensitively disrupting the determination of the rate constants. In known TIRF-analysis chambers or bio-sensor systems with a liquid interface layer of ~10 mum thickness and mass transport coefficients of 10-6-10-5 m/s it has up limitation.With the help of a TIRF-flow-through shear analyzer in which a certain volume unit of an immiscible fluid, for example an air bubble, is fed into the buffer flow, an ultra-thin liquid layer arises on the surface with a thickness of 100-200 nm, wherein interface surfaces below 10 nm thickness are technically possible.The new TIRF-flow-through shear analyzer therefore allows the generation of ultra-thin liquid layers while increasing the mass transport coefficients for proteins by 50-100-fold so that the sorption rate constants can be determined without transport limitation.
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