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Process for purifying double-stranded DNA, process for purifying double-stranded RNA and process for purifying plasmid DNA

机译:纯化双链DNA的方法,纯化双链RNA的方法和纯化质粒DNA的方法

摘要

In the present invention, there is disclosed a process for purifying double-stranded DNA wherein the process is characterized in that it comprises at least one purification stage in which a solution containing said double-stranded DNA in a mixture with other components is passed over a support on which is covalently coupled an oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix wherein said specific sequence is represented by a sequence being naturally present in the double-stranded DNA or a sequence that has been introduced artificially into the double-stranded DNA. The invention also relates to a purification process of a double-stranded RNA as well as a purification process of a plasmid DNA, wherein both the purification processes are carried out in the same manner.
机译:在本发明中,公开了一种纯化双链DNA的方法,其中该方法的特征在于它包括至少一个纯化阶段,其中使包含与其他组分混合的所述双链DNA的溶液通过在其支持物上共价偶联能够与所述DNA上存在的特定序列杂交形成三螺旋的寡核苷酸,其中所述特定序列由天然存在于双链DNA中的序列或已人工引入的序列表示进入双链DNA。本发明还涉及双链RNA的纯化方法以及质粒DNA的纯化方法,其中两种纯化方法均以相同的方式进行。

著录项

  • 公开/公告号CZ295474B6

    专利类型

  • 公开/公告日2005-08-17

    原文格式PDF

  • 申请/专利权人 CENTELION;

    申请/专利号CZ19970001858

  • 发明设计人 CROUZET JOEL;SCHERMAN DANIEL;WILS PIERRE;

    申请日1995-11-08

  • 分类号C12Q1/68;

  • 国家 CZ

  • 入库时间 2022-08-21 22:18:14

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