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Reaction mixture for determining hemoglobin in a blood sample and method for the determination of hemoglobin using a whole blood sample

机译:用于测定血样中血红蛋白的反应混合物以及使用全血样品测定血红蛋白的方法

摘要

In the present invention, there is described a reagent composition for the determination of hemoglobin in a blood sample, comprising in aqueous admixture at least one surfactant in an amount effective to hemolyze red blood cells in said sample and form micelles; a soluble inorganic cyanide salt in an amount effective to bind to heme molecules resulting from said blood cell hemolysis; and a pH buffer capable of maintaining a pH of said reagent composition at about 10.0 to about 11.2; wherein said reagent composition in admixture with said blood sample provides freedom from white blood cell interference in automated hemoglobin detection methods. Further disclosed is a method for determining hemoglobin in a whole blood sample, said method comprising: a) mixing an aliquot of said blood sample with the above-described reagent composition to form a reaction mixture; and b) analyzing said reaction mixture on an automated hematology analyzer instrument to determine hemoglobin using the above-indicated reagent composition.
机译:在本发明中,描述了一种用于测定血液样品中血红蛋白的试剂组合物,该试剂组合物在水性混合物中包含至少一种有效量的表面活性剂,该表面活性剂能有效地溶解所述样品中的红细胞并形成胶束。可溶性无机氰化物盐,其量应能有效结合所述血细胞溶血产生的血红素分子;能够将所述试剂组合物的pH维持在约10.0〜约11.2的pH缓冲剂。其中在自动血红蛋白检测方法中,与所述血液样品混合的所述试剂组合物不受白细胞干扰。还公开了确定全血样品中血红蛋白的方法,所述方法包括:a)将所述血样的等分试样与上述试剂组合物混合以形成反应混合物; b)使用上述试剂组合物在自动血液分析仪上分析所述反应混合物以确定血红蛋白。

著录项

  • 公开/公告号CZ294599B6

    专利类型

  • 公开/公告日2005-02-16

    原文格式PDF

  • 申请/专利权人 BAYER CORPORATION;

    申请/专利号CZ19980001461

  • 发明设计人 MALIN MICHAEL J.;

    申请日1998-05-12

  • 分类号G01N33/72;

  • 国家 CZ

  • 入库时间 2022-08-21 22:18:19

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