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Ratiometric fluorescent pH sensor for non-invasive monitoring

机译:比例式荧光pH传感器用于无创监测

摘要

The present invention provides ratiometric fluorescent pH sensors for non-invasive, continuous monitoring of pH in such applications as fermentation processes. The ratiometric fluorescent pH sensors comprise a fluorescent dye that exhibits a shift in excitation wavelength with a corresponding shift in pH in the local environment of said fluorescent dye. Ratiometric measurements of the emission intensities at dual excitation maxima correlate to pH. Also provided is a fluorescent dye 6-methacryloyl-8-hydroxy-1,3-pyrene disulfonic acid (MA-HPDS). Further provided are systems and methods to non-invasively and continuously monitor pH.
机译:本发明提供了比例式荧光pH传感器,用于在诸如发酵过程之类的应用中无创,连续地监测pH。比率式荧光pH传感器包括荧光染料,其在所述荧光染料的局部环境中表现出激发波长的偏移以及pH的相应偏移。双重激发最大值下发射强度的比率测量与pH相关。还提供了荧光染料6-甲基丙烯酰基-8-羟基-1,3-py二磺酸(MA-HPDS)。还提供了非侵入性和连续监测pH的系统和方法。

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