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METHOD PREPARING STREPTOMYCIN-RESISTANT VARIANTS OF ANTHRAX PATHOGEN FROM SYMBIOTIC MIXTURE OF CELLS OF BACILLUS ANTHRACIS AND SLIPPING MICROORGANISM MYXOCOCCUS XANTHUS
METHOD PREPARING STREPTOMYCIN-RESISTANT VARIANTS OF ANTHRAX PATHOGEN FROM SYMBIOTIC MIXTURE OF CELLS OF BACILLUS ANTHRACIS AND SLIPPING MICROORGANISM MYXOCOCCUS XANTHUS
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机译：从炭疽芽孢杆菌细胞的符号混合和打滑微生物黏附力黄曲霉制备抗链霉素抗性炭疽变种的方法
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摘要
FIELD: microbiology, ecology.;SUBSTANCE: invention relates to methods for preparing streptomycin-resistant variants of anthrax pathogen. Method involves stratifying membrane filters on surface of solid nutrient medium. Breeding streptomycin-resistant strain of B. anthracis with M. xanthus is carried out by mixing cell biomass of these microorganisms and seeding this mixture on surface of membrane filters stratified on surface of nutrient medium. Separate seeding of biomass B. anthracis and M. xanthus is used as control. Seeding is incubated in thermostat at 35 C for 24 h. Then replication (fingerprints) of grown populations is carried out in selective medium containing streptomycin in the concentration 100 mcg/ml and incubated colony fingerprints at 35 C for 48-72 h. Method provides detecting and preparing streptomycin-resistant variants of anthrax pathogen from symbiotic mixture of cells B. anthracis and slipping microorganism M. xanthus.;EFFECT: improved preparing method.;1 tbl, 3 ex
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机译：技术领域本发明涉及制备抗炭疽病菌的链霉素抗性变体的方法。方法包括将固体营养培养基表面的膜过滤器分层。通过混合这些微生物的细胞生物量并将该混合物播种在营养培养基表面分层的膜滤器表面上，来进行抗微生物链霉菌与黄腐杆菌的选育。将生物量炭疽杆菌和黄单胞菌的单独播种用作对照。将种子在恒温器中于35°C孵育24小时。然后在含有链霉素浓度为100 mcg / ml的选择性培养基中进行生长种群的复制（指纹图谱），并在35 C下孵育菌落指纹图谱48-72 h。该方法提供了从炭疽芽孢杆菌细胞和滑动性微生物黄腐霉菌的共生混合物中检测和制备抗炭疽病菌的链霉素变体的方法;效果：改进的制备方法。1tbl，3 ex
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