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METHOD FOR MODELING SYMBIOSIS OF MELIODOSIS PATHOGEN CELLS WITH SAPROPHYTE MYXOCOCCUS XANTHUS
METHOD FOR MODELING SYMBIOSIS OF MELIODOSIS PATHOGEN CELLS WITH SAPROPHYTE MYXOCOCCUS XANTHUS
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机译：腐生腐霉黄腐病对黑穗病菌细胞共生症的建模方法
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摘要
FIELD: microbiology, ecology. SUBSTANCE: invention relates to methods for modeling symbiosis (biocenosis) of meliodosis pathogen with saprophyte slipping microorganisms and the following detection of cells B. pseudomallei in this symbiosis. Method involves seeding cultures B. pseudomallei and M. xanthus by streak method on surface of solid nutrient medium in the definite picture. After additional culturing seeding at 34 C for 72-96 h cells of meliodosis pathogen are included into fold-pockets of M. xanthus fruit bodies. Part of B. pseudomallei cells are lysed inside of fruit bodies but some cells of meliodosis pathogen are adapted to the new habitation medium, persisted in it and propagated even so, i. e. cells show symbiosis. For detection of B. pseudomallei cells in fruit bodies of M. xanthus biomass of fruit bodies containing single cells of meliodosis pathogen presumably is cultured additionally preliminary in nutrient broth, treated with chloroform and chloroform lysate is used as donor material (i. e. DNA preparation) for carrying out the genetic transformation. Genetic transformation is carried out in minimal medium by auxotrophy marker using the univercal recipient P. pseudomallei SVBK 141 Pur. The presence of cells B. pseudomallei in fruit bodies is estimated indirectly by formation of prototrophic colonies (recombinants) in minimal medium that appear as result of genetic transformation purine-dependent cells of universal recipient with donor material. Invention provides the development of biological model of symbiosis of pathogenic microorganism (B. pseudomallei) with ubiquist wide-spread saprophyte (M. xanthus), allows to detect single cells of meliodosis pathogen in biomass of this biocenosis that can be used for investigation of ecological systems and assay of ecological valence of components. EFFECT: improved modeling method. 2 cl, 1 dwg, 3 ex
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机译：领域：微生物学，生态学。发明领域本发明涉及模拟腐生病病原体与腐生腐殖质滑动微生物共生（生物学）的方法，并随后检测该共生中假芽孢杆菌细胞。该方法涉及在确定的图片中通过条纹法在固体营养培养基的表面上播种假单胞菌和黄腐菌。在34℃下进一步培养播种72-96小时后，将旋毛病病原体细胞包括在黄腐果子实体的折叠袋中。假苹果芽孢杆菌细胞的一部分在子实体中被溶解，但是某些旋律病病原体细胞适应了新的生境培养基，并在其中持续存在并繁殖。 e。细胞显示共生。为了检测黄萎病菌的子实体中的假苹果芽孢杆菌细胞，推测将含有旋毛病病原体单细胞的子实体生物质在营养肉汤中另外进行初步培养，并用氯仿和氯仿裂解物处理作为供体材料（即DNA制剂）用于进行基因改造。使用单向受体假单胞菌SVBK 141 Pur，通过营养缺陷型标记在最小培养基中进行遗传转化。通过在基本培养基中形成原养集落（重组体）来间接估计子实体中假单胞菌在子实体中的存在，该原代集落是由具有供体物质的通用受体嘌呤依赖性细胞进行遗传转化而形成的。本发明提供了与普遍存在的广泛腐生菌（X. xanthus）共生的病原微生物（假拟芽孢杆菌）共生的生物学模型，其允许检测该生物群落的生物质中的病菌病原体的单个细胞，可用于生态学研究。系统和成分生态价的测定。效果：改进的建模方法。 2 cl，1 dwg，3 ex
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